Effects of L-carnitine on the expression of PDGF and Profilin-1 in rats with myocardial infarction by inhibiting PI3K/Akt/Sirt1 signaling pathway
Zhou Huiliang
Gan Shouyi
Feng Guangrui
Li Bin
Song Qiaoqiao
Liu Liang
Abstract：investigate the effects of L-carnitine on PI3K/Akt/Sirt1 signaling pathway and the expression of platelet-derived factor (PDGF) and prefibrin-1 (Profilin-1) in acute myocardial infarction (AMI) rats. Methods Forty-five adult Wistar rats were randomly divided into control group (sham operation group), model group and left carnitine group. The drug intervention was performed for 2 weeks after AMI modeling. Serum levels of troponin (cTnT), creatine kinase (CK-MB), PDGF, and Profilin-1 were measured by enzyme-linked immunosorbent assay. CCK-8 method and flow cytometry were used to detect the viability and apoptosis rate of myocardial cells in fine rats. Western blot and qRT-PCR were used to detect PI3K/Akt/Sirt1 signaling pathway protein levels and mRNA levels. Results The levels of serum cTnT and CK-MB in the model group were significantly higher than those in the control group (P＜0.05). The levels of serum cTnT and CK-MB in the L-carnitine group were significantly lower than those in the model group (P＜0.05). The cell viability of cardiomyocytes in the model group was significantly lower than that in the control group, and the apoptosis rate was significantly higher than that in the control group (P＜0.05). The cardiomyocyte viability in the left carnitine group was significantly higher than that in the model group, and the apoptosis rate was significantly lower. In the model group (P＜0.05). The serum PDGF of the model group was significantly lower than that of the control group and the Profilin-1 level was significantly higher than that of the control group (P＜0.05). The serum PDGF of the left carnitine group was significantly higher than that of the model group and the Profilin-1 level was significantly lower than the model. Group (P＜0.05). The levels of PI3K, Akt, Sirt1 protein and mRNA in the myocardial tissue of the model group were significantly lower than those in the control group (P＜0.05). The levels of PI3K, Akt, Sirt1 protein and mRNA in the myocardial tissue of the L-carnitine group were significantly higher than those in the model group (P＜0.05). Conclusion L-carnitine has the effect of promoting the expression of PDGF and PI3K/Akt/Sirt1, promoting myocardial proliferation and inhibiting apoptosis, and at the same time improving the oxidative stress state and down-regulating the level of Profilin-1.
Keywords：Myocardial infarctionL-carnitinePlatelet-derived growth factorProfilin-1
Publication Date：2019-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：4( 1188-1191 )