Influence of atorvastatin on membrane potential of rat aortic smooth muscle cells through up-regulating current of large conductance calcium-sensitive potassium channel
Xiao Yihui
Wei Yuejiao
Cao Yumeng
Gao Yuan.
Abstract：Objective To investigate the influencing mechanism of atorvastatin (ATV) on membrane potential of rat aortic smooth muscle cells (ASMC) through up-regulating current of large conductance calcium-sensitive potassium channel (BKca). Methods ASMC were divided into control group (normal saline), 10 μmol/L ATV group, 25 μmol/L ATV group, 50 μmol/L ATV group, 100 μmol/L ATV group and 150 μmol/L group. When BKca blocker-iberiotoxin (IBTX, 100 nmol/L) was used, ASMC membrane potential was detected by using laser scanning confocal microscope (LSCM). The potassium current density (pA/pF) of the whole ASMC was detected by using whole-cell patch clamp technique. Results The fluorescence value of ASMC membrane potential decreased in a concentration-dependent manner after the co-cultivation of ASMC and ATV. Compared with control group, the fluorescence value decreased in 100 μmol/L ATV group and 150 μmol/L group (P<0.05). IBTX reduced significantly pA/pF value in control group (P<0.05). Compared with control group, the increases of pA/pF value had statistical significance in all ATV groups (P<0.05). The changes of potassium current density had no statistical significance in 100 μmol/L ATV+IBTX group (P>0.05). Compared with 100 μmol/L ATV group, the decrease of pA/pF value had statistical significance in 100 μmol/L ATV+IBTX group (P<0.05). Conclusion ATV can induce the hyperpolarization of ASMC through up-regulating the current of BKca, and then influences the biological activity of ASMC.
Keywords：AtorvastatinAortic smooth muscle cellsMembrane potentialLarge conductance calcium-sensitive potassium channels
Publication Date：2019-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：4( 1092-1095 )