Protective effect of Netrin-1 on H9c2 injury induced by hypoxia and reoxygenation in cardiomyocytes
Liu Shuzhen
Wan Daguo
Ma Jun
Sun Jinqi
Xie Yanhui
Abstract：Objective To investigate the role of Netrin-1 in myocardial H9c2 hypoxia-reoxygenation injury and to explore its mechanism. Methods A model of H9c2 hypoxia-reoxygenation injury in cardiomyocytes was established. A/R+Netrin-1 group (transfected with pcDNA 3.1-Netrin-1), A/R+ Ctrl group (transfected with pcDNA 3.1), A/R+Netrin-1 +LY294002 group (transfected with pcDNA 3.1-Netrin-1 co-treated with inhibitor LY294002) and A/R+ Netrin-1+DMSO group (transfected with pcDNA 3.1-Netrin-1 and DMSO) were transfected by liposome method. H9c2 cells were stained; LDH, MDA and SOD were measured by ELISA; cell viability was measured by MTT assay; protein expression of Bcl-2, Bax, Cleaved caspase-3, Netrin-1 and p-AKT was measured by WesternBlot; apoptotic rate of cells were measured by cytology. Results Compared with the A/R+Ctrl group, the levels of LDH and MDA in the A/R+Netrin-1 group were significantly decreased, the SOD content was significantly increased, the cell viability was significantly increased, and the protein expression of Bax and Cleaved caspase-3 was significantly increased. The expression of Bcl-2 protein was significantly increased, the expression of Netrin-1 and p-AKT protein were significantly increased, and the apoptosis rate was significantly decreased (P<0.05). Compared with the A/R+Netrin-1+DMSO group, the LDH and MDA contents in the A/R+Netrin-1+ LY294002 group were significantly increased, the SOD content was significantly decreased, and the cell viability was significantly decreased. Bax and Cleaved caspase-3 were significantly decreased. The expression of Bcl-2 protein was significantly decreased, the expression of Netrin-1 and p-AKT protein were significantly decreased, and the apoptosis rate was significantly increased (P<0.05). Conclusion Netrin-1 can promote the hypoxia-reoxygenation myocardial cell viability, inhibit apoptosis and play a protective role by activating Akt pathway, which will provide a theoretical basis for the treatment of myocardial injury.
Keywords：Netrin-1Akt pathwayHypoxia-reoxygenation cardiomyocytesApoptosis
Publication Date：2019-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：5( 945-949 )