Mechanisms of atorvastatin reversing the effect of IL-1β on membrane potential of rat aortic smooth muscle cells
Xiao Yihui
Li Ting
Cao Yumeng
Gao Yuan
Abstract：Objective To investigate the mechanism of atorvastatin reversing the effect of IL-1β on membrane potential of rat aortic smooth muscle cells. Methods Grouping: control group, IL-1β treatment group, IL-1β+catalase (H2O2 scavenger) treatment group, IL-1β+ atorvastatin treatment group, IL-1β+atorvastatin+catalase treatment group; The H2O2 level in each group was determined by hydrogen peroxide detection kit. DiBol4 (3) was used as the membrane potential fluorescent label, and the membrane potential of each group was detected by laser confocal microscopy. The open probability NPo was used as an indicator and recorded by single channel patch clamp technique. Cell membrane BKca channel activity in each group. Results Compared with the control group, the IL-1β treatment group up-regulated the intracellular H2O2 level (P<0.05). Compared with IL-1β treatment group, IL-1β+catalase treatment group and IL-1β+atorvastatin treatment group all down-regulated H2O2 levels (P<0.05). Compared with the IL-1β+atorvastatin treatment group, there was no significant difference in the level of H2O2 between the IL-1β+atorvastatin+catalase treatment group (P>0.05). Compared with the control group, the IL-1β treatment group down-regulated the NBo of the cell membrane BKca channel. Compared with IL-1β treatment group, IL-1β+catalase treatment group and IL-1β+atorvastatin treatment group up-regulated the cell membrane BKca channel NPo (P<0.05). Compared with IL-1β+atorvastatin treatment group, there was no significant difference in NK of cell membrane BKca channel in IL-1β+atorvastatin+ catalase treatment group (P>0.05). Conclusion Atorvastatin reverses the effect of IL-1β on cell membrane potential by down-regulating the level of hydrogen peroxide in rat aortic smooth muscle cells and up-regulating the activity of large conductance calcium-sensitive potassium channels.
Keywords：AtorvastatinIL-1βAortic smooth muscle cellsMembrane potentialLarge conductance calcium-sensitive potassium channels
Publication Date：2019-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：4( 830-833 )