Expression of serum micro RNA-223 and its effect on cardiac myocyte apoptosis in patients with acute myocardial infarction
Han Minglei
Jin Weidong
Liu Zhen
Wang Cheng
Cui Jiajia
Li Yanru
Wang Xuehui
Abstract：To study the expression of serum micro RNA-223 (mi R-223) and discuss the influence of mi R-223 on cardiac myocyte apoptosis in patients with acute myocardial infarction (AMI). Methods AMI patients (n=67, AMI group) and healthy controls (n=50, control group) were chosen from Department of Cardiovascular Medicine of Xinxiang Central Hospital from Jan. 2015 to Jan. 2017. The expression level of mi R-223 was detected by using real-time fluorescence quantitative polymerase chain reaction (RT-q PCR). The influence of mi R-223 on apoptosis of human cardiac myocytes (HCM) was studied taken HCM as model. Results The expression of mi R-223 was (5.58 ±0.99) in AMI group and (0.66±0.15) in control group (P＜0.05). The results of Logistic regression analysis showed that mi R-223 was an independent risk factor of AMI. Luciferase reporter gene system verified that mi R-223 inhibited the expression of CCTN2 gene in HCM. After H2O2 induction, the apoptosis rate of HCM transfected with mi R-223-mimc increased by 95.9% compared with that of HCM transfected with mi R-223-NC. Conclusion The expression of serum mi R-223 is higher in AMI patients, and high-expressed mi R-223 can improve HCM apoptosis possibly through targeted inhibition of CCTN2 expression in HCM.
Keywords：Micro RNA-223Cardiac myocytesAcute myocardial infarctionApoptosis
Publication Date：2019-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：4( 698-701 )