Association between circulating miR-155/PDCD4 levels and severity of coronary artery disease
Lu Zhentao
Cui Silong
Dong Yancai
Abstract：Objective To investigate the changes of miR-155 and programmed death factor 4 (PDCD4) gene expression in peripheral blood CD4+ T cells of patients with coronary heart disease (CAD) and its relationship with the severity of coronary artery disease. Methods A total of 285 CAD patients who underwent coronary angiography (CAG) and were hospitalized for treatment in the Department of Cardiology, Luohe Central Hospital from June to May 2018, 2017 were selected. In addition, 50 patients with non-coronary heart disease were selected as the control group. The clinical data of different subgroups of CAD patients, the expression of miR-155 and PDCD4 mRNA in CD4+ T cells were compared. Multivariate logistic regression was used to analyze the risk factors of CAD. The receiver operating characteristic (ROC) curve was used to analyze the early diagnostic value of miR-155 and PDCD4 for severe coronary artery disease. Results The levels of triglyceride (TG), blood urea nitrogen (BUN), serum creatinine (SCr), fasting plasma glucose (FPG), CD4+ T lymphocytes miR-155/PDCD4 mRNA in CAD patients were higher than the control subjects (P<0.05). By multivariate Logistic analysis, TG, FPG, miR-155, PDCD4 were the independent risk factors of CAD (P<0.05). The expressions of miR-155/PDCD4 mRNA and Gensini scores in patients with three lesions were higher than the patients with a group of lesions or two lesions (P<0.05). The expressions of miR-155/PDCD4 mRNA and Gensini scores in patients with severe coronary artery stenosis were higher than the patients with mild or moderate coronary artery stenosis (P<0.05). The expressions of miR-155/PDCD4 mRNA in patients with severe coronary artery lesions were higher than the patients with mild or moderate coronary artery lesions (P<0.05). According to the results of ROC curve, AUCs of miR-155, PDCD4 and miR-155/PDCD4 combination were respectively 0.766 (95%CI: 0.631~0.908), 0.714 (95%CI: 0.594~0.875), 0.897 (95%CI: 0.732~0.981) (P<0.05) Conclusion The CD4+ T lymphocytes miR-155/PDCD4 could be the potential targets of diagnosis and the individual gene therapy for coronary artery disease, as the diagnostic indexes of the severity of coronary artery lesions.
Keywords：Coronary artery diseaseCoronary artery lesionsGensini scoremiR-155PDCD4
Publication Date：2019-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：5( 618-622 )