Expressions of miR-26a/miR-197 induced by high glucose promoting cell apoptosis in cardiomyocytes:a study on mechanism
Liu Zuxia
Zhang Bin
Wang Wei
Zhang Kanglin
Huang Junli
Zhu Xiaoshan
Yang Feng
Abstract：Objective To investigate the influence of Expressions of miR-26a/miR-197 induced by high glucose (HG) in cardiomyocytes on cell viability and apoptosis rate. Methods H9c2 cardiomyocytes were divided into low glucose group (LG group), LG+miRcramble group, HG+miRcramble group, HG+miR-26a inhibitor group, HG+miR-197 inhibitor group and HG+miR-26a/miR-197 inhibitor group. The expressions of miR-26a and miR-197 were detected by using RT-PCR, and cell viability and apoptosis rate were detected by using methyl thiazolyl tetrazolium test (MTT) and flow cytometry (FCM). The protein expressions of β-catenin, PCNA and Bax were detected by using Western blotting assay. The target relationship between MCL1 and miR-26a/miR-197 was determined through target gene prediction software, detection of MCL1 expression after transfection of miR-26a/miR-197 mimics/inhibitor, and detection of luciferase activity after co-transfection of miR-NC, Wt-MCL1+miR-26a mimics, Wt-MCL1+miR-197 mimics, Mut-MCL1+miR-26a mimics and Mut-MCL1+miR-197 mimics. Results The expressions of miR-26a and miR-197 induced by HG in H9c2 cardiomyocytes were significantly higher in all HG groups than those in LG group, and significantly lower in HG+miR-26a/miR-197 inhibitor group than those in other HG groups (P<0.05). The cell viability and PCNA protein expression were significantly lower, and apoptosis rate and protein expressions of β-catenin and Bax were significantly higher in HG+miRcramble group than those in LG+miRcramble group (P<0.05). The cell viability and PCNA protein expression were significantly higher in HG+miR-26a inhibitor group and HG+miR-197 inhibitor group than those in HG+miRcramble group, and lower than those in HG+miR-26a/miR-197 inhibitor group. The apoptosis rate and protein expressions of β-catenin and Bax were significantly lower in HG+miR-26a inhibitor group and HG+miR-197 inhibitor group than those in HG+miRcramble group, and higher than those in HG+miR-26a/miR-197 inhibitor group (P<0.05). MCL1 was the target gene of miR-26a and miR-197. Conclusion MiR-26a/miR-197 can promote the viability and reduce apoptosis rate of cardiomyocyte induced by HG through targeting MCL1, and the mechanism may be related to the inhibition of Wnt signaling pathway.
Keywords：MiR-26aMiR-197CardiomyocytesHigh glucoseApoptosisWnt signaling pathway
Publication Date：2019-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：5( 551-555 )