Influence of Wnt5a on cardiomyocyte oxidative damage induced by hypoxia/reoxygenation
Zhao Lihua
Yin Honglei
Lyu Fenghua
Zhang Surong
Guo Changlei
Zhang Peiyong
Abstract：Objective To discuss the influence of Wnt5a on cardiomyocyte oxidative damage induced by hypoxia/reoxygenation. Methods The human cardiomyocytes (HCM) were taken as study objects and divided into normal group, model group, siRNA control group and Wnt5a siRNA group. HCM were normally cultured in normal group, and given hypoxia/reoxygenation treatment in model group, siRNA control group and Wnt5a siRNA group. HCM were transfected with Wnt5a siRNA or siRNA control respectively in siRNA control group and Wnt5a siRNA group. The mRNA and protein levels of Wnt5a in HCM were detected by using Western blotting assay and RT-PCR, HCM proliferation activity was detected by using methyl thiazolyl tetrazolium test (MTT), and HCM apoptosis was detected by using flow cytometry. The level of MDA was detected by using thiobarbituric acid (TBA) method, activity of SOD was detected by using xanthineoxidation (XTO) method, and activity of LDH was detected by using dinitrophenylhydrazine (DNPH) method. Results The mRNA and protein levels of Wnt5a, HCM apoptosis rate and levels of MDA and LDH were significantly higher (P<0.05), and HCM survival rate and SOD level were significantly lower in model group than those in normal group (P<0.05). The mRNA and protein levels of Wnt5a, HCM apoptosis rate, levels of MDA, LDH and SOD and HCM survival rate had no significant difference between siRNA control group and model group. The mRNA and protein levels of Wnt5a, HCM apoptosis rate and levels of MDA and LDH were significantly lower (P<0.05), and HCM survival rate and SOD level were significantly higher in Wnt5a siRNA group than those in model group (P<0.05). Conclusion The expression of Wnt5a increases in hypoxia/reoxygenation HCM, and intervention of Wnt5a can reduce the oxidative damage and apoptosis of HCM induced by hypoxia/reoxygenation.
Keywords：CardiomyocytesHypoxia/reoxygenationOxidative damageWnt5a
Publication Date：2019-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：5( 465-468,473 )