Changes and clinical significance of serum miR-335 and LTBP-2 levels in patients with coronary heart disease

Chu Yanzhen; Zhang Yan'ang; Yu Linjun

doi:10.3969/j.issn.1674-4055.2019.04.14

Abstract：Objective To study the changes and clinical significance of levels of serum miR-335 and LTBP-2 in patients with coronary heart disease (CHD). Methods CHD patients (n=120) were chosen into study group and healthy controls (n=50) were chosen into control group from Chinese PLA 153rd Hospital from Mar. 2016 to Mar. 2018. The expression of miR-335 was detected by using real-time fluorescence quantitative polymerase chain reaction (RT-qPCR), and expression of LTBP-2 was detected by using enzyme-linked immunosorbent assay (ELISA). The study group was divided, according to Gensini integrals, into mild lesion group (n=36), moderate lesion group (n=43) and severe lesion group (n=41). The levels of serum miR-335 and LTBP-2 were compared and given correlation analysis in different groups. Results The level of serum miR-335 was lower, and level of serum LTBP-2 was higher in study group than those in control group (all P<0.05). The level of serum miR-335 showed a gradual descending trend, and level of serum LTBP-2 showed a gradual ascending trend in mild lesion group, moderate lesion group and severe lesion group, and the results of single-factor analysis of variance showed that the difference among all groups had statistical significance (all P<0.05). The results of Pearson correlation analysis showed that the level of serum miR-335 was negatively correlated to Gensini integrals (r=-0.683, P<0.05), level of serum LTBP-2 was positively correlated to Gensini integrals (r=0.626, P<0.05), and the level of serum miR-335 was negatively correlated to level of serum LTBP-2 (r=-0.711, P<0.05). Conclusion The level of serum miR-335 has high expression and level of serum LTBP-2 has low expression in CHD patients. The above 2 indicators can be detected jointly and used for early diagnosis and severity reviews of CHD.