Expression of microRNA-1 in acute myocardial infarction rats and its effect on cardiomyocyte apoptosis
Liu Ping
Zhao Guangxuan
Wang Huiyu
Zhao Qiang
Liu Quan
Huang Zhong
Zhang Haiqin
Su Jing
Zhang Ruibo
Liu Hailiang
Zhang Yong
Yuan Zixu.
Abstract：Objective To investigate the expression of microRNA-1 (miR-1) in acute myocardial infarction (AMI) rats and its effect on myocardial H9C2 cell apoptosis. Methods Thirty rats aged 8-10 weeks were randomly divided into normal control group, sham operation group and model group, with 10 rats in each group. In the model group, a rat model of acute myocardial infarction was established by thoracotomy of coronary artery. The rats in the sham-operated group were only ligated without coronary artery, and the rats in the normal control group were not treated. Serum-free culture were used to construct a model of ischemic H9C2 cells, H9C2 cells were divided into three groups:control group, negative group and inhibition group. The control group did not receive any treatment. The negative group was transfected with NC, and the inhibition group was transfected with miR-1 inhibitor. the expression of miR-1 in ischemic myocardium and H9C2 cells were detected by quantitative real-time PCR (qPCR). H9C2 cells apoptosis rate was measured by flow cytometry. Western blot was used to detect the expression of cysteine-containing aspartate proteinase 3 (Caspase-3) and activated Caspase-3 (Cleaved Caspase-3) protein. Results There was no significant difference in the expression of miR-1 between the normal control group and the sham operation group (P>0.05). Compared with the normal control group and the sham-operated group, the expression of miR-1 in the ischemic myocardium of the model group was significantly increased (P<0.05). There was no significant difference in miR-1 expression, apoptosis rate and expression of Cleaved Caspase-3 protein in H9C2 cells between the control group and the negative group (P>0.05). Compared with the control group and the negative group, the expression of miR-1 in the Inhibition group of H9C2 cells was significantly down-regulated (P<0.05), and the apoptosis rate and the level of Cleaved Caspase-3 protein in the cells were significantly decreased (P<0.05). Conclusion The expression level of miR-1 in the rat myocardial infarction model is increased, which has the effect of promoting cardiomyocyte apoptosis.
Keywords：Acute myocardial infarctionApoptosismiR-1Caspase-3
Publication Date：2019-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：4( 336-339 )