Detection and function of plasma exosomal microRNA in patients with heart failure
Shi Bing
Li Xiaoran
Li Junxia.
Abstract：Objective To determine the difference in expression profiles of plasma exosomal microRNA (miRNA) in patients with heart failure (HF), and find out the value of exosomal miRNA in reviewing on early diagnosis and prognosis of HF. Methods The samples of peripheral plasma exosome were extracted from HF patients (n=10) and non-HF patients (n=10) by using exosome extract kit, and then high-throughput sequencing technique was applied for plasma exosomal miRNA. The miRNA with different expressions were initially screened and identified by using RT-qPCR. The correlation between function of miRNA with different expressions and N-terminal pro-brain natriuretic peptide (NT-proBNP) was analyzed. Results There were totally 24 plasma exosomal miRNA screened by using high-throughput sequencing technique. Among them 14 plasma exosomal miRNA had up-regulated expressions and 10 had down-regulated expressions. The results of RT-qPCR were consistent with those of high-throughput sequencing technique. The functions of plasma exosomal miRNA with different expressions involved inflammatory reactions, apoptosis, proliferation and autophagy. The correlation analysis showed that miR-122-5p was correlated to NT-proBNP (r=0.537, P=0.015). Conclusion The plasma exosomal miRNA may be a new type of biomarker for auxiliary diagnosis and prognosis review of HF.
Keywords：PlasmaExosomal microRNAHeart failureHigh-throughput sequencingBiomarker
Publication Date：2019-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：3( 309-311 )