Correlation between left ventricular hypertrophy and GLUT4 in spontaneously hypertensive rats mediated by high resistinemia
Han Bin
Zhang Wei
Zeng Zhuo
Zhou Yuliang
Zheng Zhipeng
Li Zhichang.
Abstract：Objective To observe the effect of high resistinemia on the expression of glucose transporter 4 (GLUT4) in hypertrophic myocardium of spontaneously hypertensive rats. Methods Thirty-six 10-week-old hypertensive rats (male) were randomly divided into a virus group and a control group (18 in each group). The virus group was continuously injected with the recombinant adenovirus carrying the resistin gene through the tail vein, while the control group was continuously injected with the same volume of normal saline through the tail vein. The virus group and the control group were randomly divided into the first week group, the fourth week group, and the eighth week group, respectively, and sacrificed in the corresponding weeks. The fasting insulin level of each group was measured, the left ventricular mass index (LVMI) was calculated, the cardiomyocytes and arrangement were changed by HE staining, the resistin level was detected by ELISA, and the expression of GLUT4 in myocardial tissue was detected by Western blot. Results Serum resistin and insulin levels in the virus group were significantly higher in the first week and the fourth week than in the control group (P<0.05). LVMI was significantly higher in the 4th week and the 8th week than in the control group (P<0.05). GLUT4 levels were significantly lower in the first week, the fourth week, and the eighth week than in the control group, and LVMI was negatively correlated with GLUT4 levels. Conclusion High level of serum resistin could cause left ventricular hypertrophy in spontaneous hypertension rats, which may be related with down-regulation of GLUT4 levels in myocardium.
Keywords：ResistinSpontaneously hypertensive ratsLeft ventricular hypertrophyGLUT4
Publication Date：2019-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：4( 301-304 )