Influence of up-regulation of PTEN expression on proliferation and migration of cardiac fibroblasts induced by angiotensin Ⅱ

Feng Xiaorong; Chen Siyu; Zhang Suilong; Huang Chunchun

doi:10.3969/j.issn.1674-4055.2019.02.08

Abstract：Objective To investigate the influence of up-regulation of PTEN expression on proliferation and migration of cardiac fibroblasts (CFB) induced by angiotensin Ⅱ (Ang Ⅱ). Methods CFB were divided into blank control group (CFB without treatment), Ang Ⅱ group (CFB treated with Ang II), Ang Ⅱ+NC group (CFB treated with Ang II after transfection of pcDNA3.1) and Ang Ⅱ+PTEN group (CFB treated with Ang Ⅱ after transfection of pcDNA3 and 1-PTEN). The expression of PTEN was detected by using Western blotting assay after CFB transfected with liposome 2000 and treated with Ang Ⅱ. The proliferation of CFB was detected by using MTT, and periodic changes of CFB were measured by using flow cytometer. The ability of migration was detected by using Transwell chamber test, and content of collagen I and collagen Ⅲ were detected by using ELISA. Results The expression of PTEN and G0/G1 phase CFB ratio decreased, and percentages of S phase CFB and G2/M phase CFB, ability of proliferation and migration of CFB and content of collagen I and collagen III increased in Ang Ⅱ group, Ang Ⅱ+NC group and Ang Ⅱ+PTEN group compared with blank control group. The expression of PTEN and G0/G1 phase CFB ratio increased, and S phase CFB and G2/M phase CFB, ability of proliferation and migration of CFB and content of collagen Ⅰ and collagen Ⅲ decreased in Ang Ⅱ+PTEN group compared with Ang Ⅱ group. The difference had no statistical significance between Ang Ⅱ group and Ang Ⅱ+NC group. Conclusion The expression of PTEN decreases in CFB induced by Ang Ⅱ, and up-regulation of PTEN expression can inhibit the proliferation and migration of CFB induced by Ang Ⅱ and synthesis of collagen.