Influence of P-selectin gene polymorphism on platelet inhibition rate, MA-ADP and platelet activation indexes in patients with coronary heart disease after PCI
Yin Fuyu
Lu Xin
Wu Xiaopeng
Abstract：Objective To analyze the relationship among P-selectin gene polymorphism, platelet inhibition rate, maximum amplitude-adenosine diphosphate (MA-ADP) of blood clots and platelet activation indexes after clopidogrel intake in patients with coronary heart disease (CHD) undergone PCI.Methods CHD patients (n=374) with success PCI were chosen from Xidian Group Hospital from Mar.2015 to Dec.2016, and genotypes of P-selectin gene at sites of-2123G/C, -1969G/A and-1817T/C were detected by using PCR-RELP in all patients.The platelet inhibition rate and MA-ADP were detected by using thrombelastogram (TEG), and levels of vasodilatorstimulated phosphoprotein (VASP), soluble CD40(sCD40) and CD62p were detected by using flow cytometer.Results The genotypes of P-selectin gene at sites of-2123G/C, -1969G/A and-1817T/C were accorded with Hardy-Weinberg equilibrium rule (P＞0.05) in subjects.Platelet inhibition rate and VASP level were lower, and levels of MA-ADP, sCD40 and CD62p were higher in patients carried CC genotype at-2123G/C site than those in patients carried GG genotype (P＜0.05).But platelet inhibition rate, VASP, MA-ADP, sCD40 and CD62p levels had no statistical difference in patients carried different genotypes between-1969G/A site and-1817T/C site (P＞0.05).The results of multi-factor COX risk ratio regression model analysis showed that CC mutant genotype at-2123G/C site was still an independent influencing factor of clopidogrel resistance (P＜0.05).Conclusion P-selectin gene polymorphism can influence platelet inhibition rate, MA-ADP and platelet activation indexes, and CC genotype at-2123G/C site is correlated to clopidogrel resistance.
Keywords：Clopidogrel resistancePercutaneous coronary interventionP-selectinSingle nucleotide polymorphismPlatelet activation
Publication Date：2018-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：5( 1523-1526,1533 )