Influence of neuregulin-1β on heart function and expressions of angiopoietin 2, cannabinoid receptor 1 and protein kinase C in rats with chronic heart failure
Yu Zhenwei
Wang Xuehui
Abstract：Objective To analyze the influence of neuregulin-1βon heart function and expressions of angiopoietin 2(Ang2), cannabinoid receptor 1(CB1) and protein kinase C (PKC) in rats with chronic heart failure (CHF).Methods Male SD rats (n=30) were randomly divided into normal group, model group and neuregulin-1βgroup (each n=10).The rat model of CHF was established by injection of doxorubicin (20 mg/kg) in caudal vein, and cardiac ultrasonography was used to confirm the model.After success modeling, neuregulin-1βgroup was given neuregulin-1β (10μg/kg·d) in caudal vein for 1 week, and normal group and model group were given isometric normal saline.All groups were given examinations of echocardiography and hemodynamics on the 9th week, and myocardial tissue samples were collected for pathological detection after HE staining.The content of PKC was detected by using Western blotting assay, and relative expressions of Ang2 and CB1 were detected by using immunohistochemistry technique in 3 groups.Results The indexes of echocardiography showed that rat model of CHF was successfully established in model group and neuregulin-1βgroup on the 8th week.The indexes of echocardiography of LVEDd and LVESd decreased significantly (P＜0.05), and LVFS and LVEF increased significantly (P＜0.05) in neuregulin-1βgroup compared with model group after intervention for 1 week.The hemodynamic indexes of LVSP, +dp/dtmax and-dp/dtmax increased significantly (P＜0.05), and LVEDP decreased significantly (P＜0.05) in neuregulin-1βgroup.The relative content of PKC decreased significantly (P＜0.05), and scores of positive regions of Ang2 and CB1 decreased significantly (P＜0.05).Meanwhile the pathological results showed that myocardial lesions were milder in neuregulin-1βgroup compared with model group, except of some myocardial edema, no significant myocardial necrosis, inflammatory infiltration and obvious vascular congestion and dilatation were observed.Conclusion The intervention of neuregulin-1βcan significantly ameliorate myocardial lesions in rats, and the mechanism may be related to the down-regulation of PKC, Ang2 and CB1 expressions.
Keywords：Neuregulin-1βChronic heart failureAngiopoietin 2Cannabinoid receptor 1Protein kinase CRats
Publication Date：2018-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：5( 1492-1495,1499 )