Influence of breviscapine on myocardial apoptosis and NF-kB pathway signaling molecules (α7nAChR, p65 and IkB-α) in rats with myocardial ischemia-reperfusion injury
Ding Dan
Jiao Lihua
Wang Xuechen
Liu Zhenyu
Fan Lihua
Li Qinghai
Abstract：Objective To observe the influence of breviscapine on myocardial apoptosis and NF-kB pathway signaling molecules (α7nAChR, p65 and IkB-α) in rats with myocardial ischemia-reperfusion (IR) injury.Methods SD rats (n=40) were randomly divided into sham-operation group, IR group, low-dose breviscapine group (25 mg/kg·d) and high-dose breviscapine group (50 mg/kg·d, each n=10).The IR model was established in all 4groups.The breviscapine groups were given intraperitoneal injection of breviscapine continuously for 1 week before PCI or CABG, and sham-operation group and IR group were given injection of normal saline in the same dose.After rats being sacrificed, the heart samples were collected for detecting myocardial infarct size.The apoptotic index of cardiomyocytes was detected by using TUNEL, and protein expressions ofα7nAChR, p65 and IkB-αwere detected by using Western blotting assay.Results The myocardial infarct size, apoptotic index of cardiomyocytes and IkB-αprotein increased significantly (P＜0.01), and p65 andα7nAChR proteins decreased significantly (P＜0.05) in IR group compared with sham-operation group.The myocardial infarct size, apoptotic index of cardiomyocytes and IkB-αprotein decreased significantly (P＜0.01), and p65 andα7nAChR proteins increased significantly (P＜0.05) in high-dose breviscapine group and low-dose breviscapine group compared with IR group in a dose-dependence manner.Conclusion The pretreatment of breviscapine can effectively relieve myocardial IR injury and reduce cardiomyocyte apoptosis, and the mechanism may be related to the up-regulation ofα7nAChR for inhibiting cardiomyocyte apoptosis via NF-kB pathway and playing a role of myocardial protection.
Keywords：BreviscapineMyocardiumIschemia-reperfusionPathway of nuclear factor-κBCholinergic anti-inflammatory pathwayRats
Publication Date：2018-12-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：5( 1480-1483,1487 )