Metabolism disorder of DDAH1 and ADMA in patients with hypertension accompanied by pulmonary arterial hypertension
Zhu Xiaoqin
Deng Rensheng
Liu Changzhao
Hua Xiaofang
Abstract：Objective To study the changes and clinical value of plasma dimethylarginine dimethylaminohydrola-1 (DDAH1) and asymmetric dimethylargini (ADMA) in patients with hypertension accompanied by pulmonary arterial hypertension (PAH). Methods The patients with hypertension accompanied by PAH (n=72, hypertension+PAH group), patients with only PAH (n=43, PAH group), patients with only hypertension (n=80, hypertension group) and healthy controls (n=78, healthy group) were chosen from Department of Cardiovascular Medicine of Central Hospital of Enshi Tujia and Miao Autonomous Prefecture in Hubei Province from Mar. 2016 to Sept. 2017. The levels of plasma ADMA and DDAH1 were detected by using enzyme-linked immunosorbent assay (ELISA) in all groups. The levels of plasma ADMA and DDAH1 were analyzed by using one-way ANOVA in all groups, and sensitivity and specificity of ADMA and DDAH1 in diagnosis of hypertension accompanied by PAH were analyzed by using receiver operating characteristic curve (ROC). The odds ratio (OR) and 95% confidence interval (95%CI) were calculated by using Logistic regression analysis in predicting risk of hypertension accompanied by PAH based on levels of plasma ADMA and DDAH1. Results The level of ADMA was significantly higher in hypertension+PAH group and hypertension group than that in PAH group and healthy group, and significantly higher in hypertension+PAH group than that in hypertension group (P<0.05). The activity of DDAH1 was significantly lower in hypertension+PAH group than that in hypertension group, PAH group and healthy group (P<0.05). The difference in activity of DDAH1 had no statistical significance among hypertension group, PAH group and healthy group (P>0.05). The results of Logistic regression analysis showed that, after correcting age, sex, BMI, diabetes, smoking and blood fat level, the higher level of ADMA (OR=2.32, 95%CI: 1.76~3.14, P<0.001) and lower activity of DDAH1 (OR=0.61, 95%CI: 0.13~0.87, P<0.001) were still correlated to the occurrence of hypertension accompanied by PAH. The results of ROC analysis showed that AUC of combined ADMA and DDAH1 was 0.924 (95%CI:0.868~0.979,P<0.001), sensitivity was 85.5% and specificity was 98.6% in differentiating hypertension+PAH group and non-hypertension+PAH group (hypertension group, PAH group and healthy group). Conclusion The levels of plasma ADMA and DDAH1 may by latent biomarkers for early diagnosis of hypertension accompanied by PAH.
Keywords：Hypertension accompanied by pulmonary arterial hypertensionDimethylarginine dimethylaminohydrola-1Asymmetric dimethylarginiEarly diagnosis
Publication Date：2018-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：5( 1402-1406 )