Effect of p38MAPK in vascular endothelial cell injury induced by oxidized low density lipoprotein
Hao Dan
Zhou Daliang
Yu Xiying
Zhang Chunru
Wei Lin
Abstract：Objective To investigate the effect of p38 mitogen-activated protein kinases (p38MAPK) in vascular endothelial cell injury induced by oxidized low density lipoprotein (ox-LDL). Methods Human umbilical vein endothelial cells 12 (HUVEC-12) were divided into control group, ox-LDL group, and test groups 1, 2 and 3. The ox-LDL group and test groups 1, 2 and 3 were given inducing treatment with ox-LDL, and then test groups 1, 2 and 3 were given pretreatment with p38MAPK inhibitor in different doses respectively (5 μmol/L, 15 μmol/L, 30 μmol/L). The phosphorylation level of p38MAPK was determined by using Western blotting assay in all groups. The levels of lactic dehydrogenase (LDH), malondialdehyde (MDA) and superoxide dismutase (SOD) were detected respectively by using dinitrophenylhydrazine (DNPH) method, thiobarbituric acid (TBA) method and xanthineoxidation (XTO) method. The apoptosis of HUVEC-12 was determined by using flow cytometry (FCM), and level of Cleaved Caspase-3 was detected by using Western blotting assay in all groups. The content of nitric oxide (NO) was detected by using chromatoptometry, and content of intercellular adhesion molecule-1 (ICAM-1) and interleukin-1β (IL-1β) were detected by using ELISA. Results The phosphorylation level of p38MAPK was significantly higher in ox-LDL group than that in control group (P<0.05), and was significantly lower in test groups 1, 2 and 3 than that in ox-LDL group (P<0.05). The content of LDH and MDA increases significantly, SOD level decreased significantly, HUVEC-12 apoptosis mediated by Caspase-3 increased significantly, NO content decreased significantly, and content of ICAM-1 and IL-1β increased significantly in ox-LDL group compared with control group (P<0.05). The content of LDH and MDA decreases significantly, SOD level increased significantly, HUVEC-12 apoptosis mediated by Caspase-3 decreased significantly, NO content increased significantly, and content of ICAM-1 and IL-1β decreased significantly in test groups 1, 2 and 3 compared with ox-LDL group (P<0.05). Conclusion The phosphorylation of p38MAPKin vascular endothelial cells can be induced by ox-LDL, and oxidative damage of vascular endothelial cells induced by ox-LDL can be relieved and apoptosis can be decreased after inhibiting p38MAPK.
Keywords：Vascular endothelial cellsOxidized low density lipoproteinp38 Mitogen-activated protein kinasesOxidative damageApoptosis
Publication Date：2018-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：5( 1343-1347 )