Influence and mechanism of aldehyde dehydrogenase 2 on ischemia reperfusion injury and apoptosis of myocardial cells in rats
Yang Li
Zhang Junfeng
Yang Wenlong
Zhang Tiantian
Abstract：Objective To observe the influence of aldehyde dehydrogenase 2 (ALDH2) on ischemia reperfusion injury (IRI) and apoptosis of myocardial cells in rats and study potential mechanism. Methods Healthy male SD rats (n=40) were chosen and randomly divided into sham-operation group, agonist (ethanol)+sham-operation group, IRI group and agonist+IRI group (each n=10). Ethanol injection was given to rats and IRI model was established. Only opening chest without anterior descending branch ligation was given to sham-operation group. There were 5 rats were chosen from each group for detecting area of myocardial infarction (MI), and left ventricular tissue was collected from other 5 rats in each group for HE staining. The protein expressions of ALDH2, JNK, p-JNK, Bcl-2 and Bax were detected by using Western blotting assay, and apoptosis was detected by using TUNEL method. Results The proportion of MI was higher in IRI group than that in agonist+IRI group [(52.51±7.12)% vs. (24.10 ±5.37)%, P<0.05]. The structure of heart was normal in sham-operation group and agonist+sham-operation group, and injured seriously in IRI group. The myocardial injury was milder in agonist+IRI group. The expressions of ALDH2 and Bcl-2 were lower in IRI group than those in agonist+IRI group [(25.28±3.92) vs. (37.42±7.27), (22.24±3.20) vs. (32.04±4.86), all P<0.05]. The expression of Bax was higher [(131.88±16.88) vs. (114.50± 5.15)], and expression of Bcl-2/Bax was lower in IRI group than those in agonist+IRI group [(0.17±0.04) vs. (0.28 ±0.05), P<0.05]. The protein expressions of JNK and p-JNK was higher [(193.03±3.98) vs. (154.17±9.82), (229.16±11.17) vs. (165.57±9.30)], and ratio of p-JNK to JNK (p-JNK/JNK) was higher in IRI group than those in agonist+IRI group (all P<0.05). The rate of apoptosis was higher in IRI group and agonist+IRI group than that in sham-operation group and agonist+sham-operation [(38.36±9.08)% and (16.33±2.29)% vs. (4.76±1.41)% and (5.19±0.62)%]. The expression of ALDH2 was negatively correlated to rate of apoptosis (r=-0.799, P<0.01) and p-JNK/JNK (r=-0.752, P<0.01). Conclusion ALDH2 may inhibit apoptosis of myocardial cells, alleviate IRI and play a myocardial protective role through inhibiting activity of JNK signal pathway.
Keywords：Aldehyde dehydrogenase 2Ischemia reperfusion injuryApoptosisBcl-2BaxJNKP-JNK
Publication Date：2018-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：5( 461-465 )