Changes of expressions of myocardial AQP1 and HIF-1α and relationship between them and myocardial edema in rats with myocardial infarction
Han Yanhui
Wang Yan
Jia Jingjing
Wang Honglei
Zhu Jihong
Lai Lihong
Li Ying
Abstract：Objective To observe the expressions of aquaporin (AQP); hypoxia-inducible factor 1α (HIF-1α) in myocardial infarction tissue, and discuss the correlation between them and myocardial edema in rat model of myocardial infarction (MI). Methods SD rats (n=48) were randomly divided into MI group and sham-operation group (each n=24). The rat model of MI was established by using ligation of left anterior descending coronary artery in MI group, and only thoracotomy without ligation in sham-operation group. The rats were killed in batches to collect left ventricular myocardial tissue after modeling for 12 h, 24 h, 48 h and 72 h. The protein expressions of AQP1 and HIF-1α were detected by using ELISA. The mRNA expressions of AQP1 and HIF-1α were detected by using real-time fluorescence quantitative polymerase chain reaction (RT-PCR). The water content in myocardial tissue was detected by using dry-wet method. Results The protein and mRNA expressions of AQP1 and HIF-1α increased significantly in MI group than those in sham-operation group (all P<0.05). The protein and mRNA expressions of AQP1 and HIF-1α increased in a time gradient in MI group after operation for 72 h, and difference at all time points had statistical significance (all P<0.05). Water content increased gradually in MI group after operation for 12 h, 24 h, 48 h and 72 h, and increased compared with sham-operation group (all P<0.05). The protein and mRNA expressions of AQP1 were positively correlated to myocardial water content (r=0.507, P=0.010;r=0.585,P=0.008) and protein and mRNA expressions of HIF-1α were positively correlated to myocardial water content (r=0.408,P=0.025;r=0.486,P=0.017). Conclusion AQP1 and HIF-1α show high expressions in myocardial tissue, and are correlated closely to severity of myocardial edema in MI rats.
Keywords：Acute myocardial infarctionMyocardial edemaAquaporinHypoxia-inducible factor 1αRats
Publication Date：2018-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：4( 418-421 )