Regulating effect of paclitaxel-hirudin compound on NF-κB p65 and its downstream inflammatory factors during HCASMC inflammatory activated process induced by lipopolysaccharide
Li Hongmei
Wang Xian
Abstract：Objective To investigate the influence of stent coating drug, paclitaxel-hirudin compound, on expressions of NF-κB p65, a nuclear transcription factor, and its downstream inflammatory factors during inflammatory activated process of human coronary artery smooth muscle cells (HCASMC) induced by lipopolysaccharide (LPS). Methods HCASMC were divided into blank control group (normal HCASMC), LPS model group (intervened by LPS), high-dose paclitaxel-hirudin compound group (intervened by 1 μmol/L paclitaxel+0.2 mg/mL hirudin+LPS) and low-dose high-dose paclitaxel-hirudin compound group (intervened by 1 μmol/L paclitaxel+0.0125 mg/mL hirudin+LPS). The expressions of supernatant tumor necrosis factor-α (TNF-α), interleukin-6 (IL-6) and interleukin-1β (IL-1β) were detected by using ELISA. The mRNA expressions of NF-κB p65, TNF-α, IL-6 and IL-1β were detected by using Q-PCR, and protein expression of NF-κB p65 was detected by using Western blotting assay. Results The mRNA expressions of NF-κB p65, TNF-α, IL-6 and IL-1β increased in LPS model group compared with blank control group (all P<0.05), and all of them were lower in high-dose and low-dose paclitaxel-hirudin compound groups than those in LPS model group (all P<0.05). The protein expression of NF-κB p65 was significantly higher in LPS model group than that in blank control group, and was lower in high-dose and low-dose paclitaxel-hirudin compound groups than that in LPS model group, and the decrease was more significant in high-dose paclitaxel-hirudin compound group. Compared with blank control group, levels of TNF-α, IL-1β and IL-6 increased in LPS model group (all P<0.05). Compared with LPS model group, levels of TNF-α, IL-1β and IL-6 decreased significantly in high-dose and low-dose paclitaxel-hirudin compound groups (all P<0.05). The decrease of IL-1β was more significant in high-dose paclitaxel-hirudin compound group compared with low-dose paclitaxel-hirudin compound group (P<0.05). Conclusion Paclitaxel-hirudin compound has significant inhibitory effect on NF-κB p65 activation, down-regulates effectively transcriptional activity of NF-κB p65, and inhibits significantly expressions of downstream inflammatory factors-TNF-α, IL-6 and IL-1β during HCASMC inflammatory activated process induced by LPS.
Keywords：Paclitaxel-hirudin compoundStent coating drugLipopolysaccharideNF-κB p65 signal pathwayHuman coronary artery smooth muscle cells
Publication Date：2018-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：5( 203-206,217 )