The significance of NADPH oxidases expression in adriamycin induced cardiotoxicity
Wang Zhen
Wang Menglong
Liu Jianfang
Ye Jing
Xu Yao
Jiang Huimin
Ye Di
Wan Jun
Abstract：Objective To explore the significance of NADPH oxidases expression in Adriamycin (ADR) induced cardiotoxicity. Methods 40 male C57BL/6J mice were randomly divided into control group and model group, each group had 20 mice. The model group was given intraperitoneal ADR (20 mg/kg) in one time, while the control group was given equivalent saline (20 mg/kg) in the same way. On the 5th day, all the mice were killed to measure and record their body weight, heart weight and ratio of tibia length to heart weight. Meanwhile, the myocardial apoptosis was examined using terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL) assay. The expression of mRNA and protein in Nox2 and Nox4 were measured by RT-PCR assay and western blotting in mouse myocardium. Results Compared with control group, the body weight, heart weight and ratio of tibia length to heart weight of mice in the model group with ADR treatment got significant reduction (P<0.05);TUNEL staining results showed that the apoptosis rate of myocardial tissue increased significantly in model group (P<0.05). RT-PCR and Western blotting show that the expression of Nox2 and Nox4 in ADR group mice were significantly higher than those in control group (P<0.05). Conclusion In the model of ADR-induced cardiotoxicity, the expression of Nox2 and Nox4 is significantly higher than that in control group, which indicated that Nox might be a potential therapeutic target for cardiotoxicity induced by ADR.
Keywords：NADPH oxidasesOxidative stressAdriamycinApoptosis
Publication Date：2018-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：4( 164-166,169 )