Effect of simvastatin on the expression of p53 and caspase-3 after ischemia-reperfusion injury in rats
Jing Jiao
Zhang Yongzhong
Ma Hailing
Yan Wensheng
Zhang Yuqing
Jiao Zongwei
Abstract：Objective To observe the effect of simvastatin on the apoptosis of myocardial tissue after renal ischemia-reperfusion injury in rats. Methods a total of 36 healthy male SD rats were randomly divided into 3 groups: ① sham operated group; ② renal ischemia reperfusion group; ③ simvastatin group. The simvastatin group were administered 20 mg/kg/d of simvastatin for 2 weeks, the sham and ischemic groups were administered the same amount of saline every day. After 2 weeks, the model of renal ischemia-reperfusion injury was duplicated. First of all, 10% chloral hydrate was used for intraperitoneal anesthesia. Then the abdominal cavity was opened and the bilateral renal arteries and veins were clamped in renal ischemia reperfusion group and simvastatin group. After 45 minutes, the blood flow was recovered and the abdominal wall was sutured. The sham operation group received laparotomy without vascular clamping. After 24 hours the abdominal aorta was taken from each group and the heart was removed. Creatinine (Scr), urea nitrogen (BUN) in serum, the content of malondialdehyde (MDA) in myocardium, the activity of lactate dehydrogenase (LDH), creatine kinase (CK) and superoxide dismutase (SOD) and the expression level of p53 and caspase-3 were measured. Results Compared with sham operation group, the contents of Scr, BUN and MDA in ischemic group were increased (P<0.05), the activity of LDH and CK were increased (P<0.05), and the activity of SOD was decreased (P<0.05). Compared with the ischemia reperfusion group, the content of SCr, BUN and MDA in simvastatin group were decreased (P<0.05), and the activity of SOD was increased (P<0.05). The activity of LDH and CK were decreased (P<0.05). Compared with the sham operated group, expression of p53 and caspase-3 were increased in the renal ischemia reperfusion group than those in sham operated group and simvastatin group. Conclusion Simvastatin has a protective effect on myocardial ischemia reperfusion injury in rats, and the protective mechanism may be related to the elimination of free radicals by simvastatin and reduce the expression of p53 and caspase-3 protein.
Keywords：Ischemia-reperfusionSimvastatinp53caspase-3
Publication Date：2018-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：4( 23-26 )