Expressions and clinical significance of plasma microRNA-1 and urothelial carcinoma associated 1 gene in patients with acute myocardial infarction
Hu Longgang
An Yi
Abstract：Objective To detect the expressions of plasma microRNA-1 (miR-1) and urothelial carcinoma associated 1 (UCA1) gene in patients with acute myocardial infarction (AMI), and discuss their clinical significance. Methods AMI patients (n=67, male 39, female 28, aged from 39 to 76 and average age=56.3±8.1) were chosen into patient group from the Cardiovascular Hospital affiliated to Qingdao University from Mar. 2015 to Dec. 2016. At the same time, health controls (n=50, male 29, female 21, aged from 42 to 75 and average age=54.6±9.2) were chosen into control group. The plasma samples were collected from AMI patients at different time points (2 h, 8 h, 12 h, 24 h, 7 d and 14 d after hospitalization), and expressions of miR-1 and UCA1 gene were detected by using real-time fluorescence quantitative polymerase chain reaction (RT-PCR).Results The expression of plasma miR-1 was higher, and expression of UCA1 gene was lower in patients group than those in control group (allP<0.05). In patients group, the expression of plasma miR-1 showed an ascending trend after hospitalization, reached the highest after 24 h and then showed a descending trend. The expression of plasma miR-1 was higher in patient group than that in control group at time points of 2 h and 14 d (allP<0.05). The expression of plasma UCA1 gene showed a descending trend, reached the lowest after 24 h and then showed an ascending trend in patients group. The expression of plasma UCA1 gene was lower in patients group than that in control group at time points of 2 h and 14 d (allP<0.05). The expression of plasma miR-1 was negatively correlated to expression of plasma UCA1 gene in patient group (r=-0.497,P<0.05). The expression of plasma miR-1 was negatively correlated to expression of plasma UCA1 gene at time points of 2 h (r=-0.498), 8 h (r=-0.492), 12 h (r=-0.531), 24 h (r=-0.596), 7 d (r=-0.422) and 14 d (r=-0.495, allP<0.05).Conclusion The expressions of plasma miR-1 and plasma UCA1 gene are abnormal in AMI patients, and they were negatively correlated to each other. The combined detection of miR-1 and UCA1 gene can be taken as important biomarker for early diagnosis of AMI.
Keywords：Acute myocardial infarctionMicroRNA-1Urothelial carcinoma associated 1 geneCorrelationBiomarkers
Publication Date：2017-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：4( 1468-1470,1477 )