Influence of hydrogen sulfide on inflammatory factors after myocardial ischemia-reperfusion injury

Huang Yanni; Zhao Xiangxiang; Zhang Yali; Cheng Gong

doi:10.3969/j.issn.1674-4055.2017.11.23

Abstract：Objective To observe the influence of hydrogen sulfide (H2S) on myocardial ischemia-reperfusion injury. Methods SD rats (n=24) were randomly divided into groups A, B and C (each n=8), and model of Langendorff cardiac perfusion in vitro was established. The groups were given, respectively, perfusion of KH solution, KH solution+NaHS (prepared with HCl and Na2S just before using, 1μM) and STH solution. At the time points of T0 (30 min before the operation), T1 (15 min after asystole), T2 (30 min after asystole), T3 (15 min after heart resuscitation) and T4 (30 min after heart resuscitation), the expressions of hypoxia inducible factor-1α (HIF-1α), interleukin-6 (IL-6), interleukin-10 (IL-10) and tumor necrosis factor-α (TNF-α) in perfusion solution were detected by using enzyme-linked immunosorbent assay (ELISA). H9C2 myocardial cells were divided into groups 1, 2 and 3, given, respectively, KH solution, KH solution+NaHS and STH solution, stored for 6 h and then recovered to room temperature. The expressions of HIF-1α, IL-6, IL-10 and TNF-α in cell supernatant were detected by using ELISA. Results The expression of HIF-1α decreased in all groups after asystole compared with before (all P<0.05). The expression of HIF-1α increased in group B compared with group A and group B at time points of T2, T3 and T4 (all P<0.05). The expressions of IL-6, IL-10 and TNF-α increased in all groups after asystole compared with before (all P<0.05). The expressions of IL-6, IL-10 and TNF-α were lower in group B than those in group A and group C at time points of T2, T3 and T4 (all P<0.05). The expression of HIF-1α increased significantly, and expressions of IL-6, IL-10 and TNF-α decreased significantly in group 2 compared with group 1 and group 3 (all P<0.05). Conclusion H2S can reduce inflammatory reactions, improve expression of HIF-1α and play a role of protecting myocardium in rats with myocardial ischemia-reperfusion injury.