Regulative effect of Luofengning Fang I on system of thrombomoduline-activated protein C-endothelial protein C receptor in rats with myocardial infarction
Sun Tian
Wang Xian
Abstract：Objective To investigate the regulative effect of Luofengning Fang I on system of thrombomoduline-activated protein C-endothelial protein C receptor (TM-APC-EPCR) in rats with myocardial infarction (MI). Methods SD rats were randomly divided into sham-operation group (n=7), model group (n=8), Luofengning Fang I group (n=7), combined therapy group (n=8) and captopril group (n=8). After modeling of MI, Luofengning Fang I group and combined therapy group were given decoction of Luofengning Fang I [contained 4.81 g/(kg. d) of crude medicinal], captopril group and combined therapy group were given captopril tablets [7.5 mg/(kg. d)], and sham-operation group and model group were the same volume of distilled water twice a day for 4 weeks. The gene and protein expressions of TM, APC and EPCR in peripheral tissue of MI were detected in all groups after 4 weeks. Results The levels of EPCR-mRNA, TM-mRNA, serum EPCR (sEPCR) and serum TM (sTM) increased significantly (P<0.05), and protein expressions of EPCR and TM and APC level decreased (P<0.05) in model group compared with sham-operation group. The levels of EPCR-mRNA and TM-mRNA decreased (P<0.05), and protein expression of EPCR increased (P<0.05) in Luofengning Fang I group and combined therapy group compared with model group. The level of serum APC increased in Luofengning Fang I group and combined therapy group compared with model group (P<0.05). Conclusion Luofengning Fang I can relieve the injury of EPCR and TM, inhibit compensatory increases of TM-mRNA and EPCR-mRNA, reduce levels of sEPCR and sTM, and improve APC level.
Keywords：Myocardial infarctionLuofengning Fang IThrombomodulineActivated protein CEndothelial protein C receptorRats
Publication Date：2017-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：4( 1342-1345 )