Effect of miR-155 gene knockout in atherosclerosis rats treated with simvastatin

Huang Shan; Meng Qingwen; Yang Yang; Tan Haitao

doi:10.3969/j.issn.1674-4055.2017.09.13

Abstract：Objective To investigate the effect of miR-155 gene knockout on the formation of atherosclerosis in rats and the effect of simvastatin on atherosclerosis in rats. Methods 20 male SD rats with miR-155 knockout were used as experimental group. Vitamin D3 was injected intraperitoneally and fed with high fat diet to rats. After the model was completed, the experimental group was randomly divided into two groups: experimental modeling group and experimental treatment group, each group has 10 rats. The rats in the experimental treatment group were given a certain dose of simvastatin . The experimental modeling group was given the same amount of saline.20 healthy male SD rats were randomly divided into normal control group and normal treatment group. 10 rats in each group, Normal treatment group was injected with the same amount of normal saline and fed as same as experimental treatment group. Normal control group was given the same amount of saline. HE staining was used to observe the damage of aorta in four groups; The serum levels of total cholesterol (TC), triglyceride (TG), low density lipoprotein (LDL) and high-density lipoprotein (HDL) were measured; QRT-PCR was used to detect the expression of vascular cell adhesion molecule-1 (VCAM-1), interleukin-6 (IL-6), monocyte chemoattractant protein- 1 (MCP-1) mRNA expression in different groups. Results Compared with the normal control group, the aorta of the model group showed typical AS plaque formation. Compared with the normal control group, the levels of TC, TG and LDLC in the experimental model group, experimental treatment group and normal treatment group were significantly higher than those in the normal model group, the difference was statistically significant (P<0.05). Compared with the experimental model group, the levels of TC, TG and LDLC in the normal treatment group were significantly lower (P<0.05). Compared with the normal treatment group, the levels of TC, TG and LDLC in experimental treatment group were significantly decreased and the level of HDLC was significantly increased (P<0.05). Compared with the normal control group, the mRNA expression levels of VCAM-1, IL-6 and MCP-1 in the experimental model group,experimental treatment group and normal treatment group were significantly higher than those in the normal control group (P<0.05). Compared with the experimental model group, the expression of VCAM-1, IL-6 and MCP-1 mRNA in the normal treatment group was significantly lower(P<0.05). Compared with the normal treatment group, the mRNA expression levels of VCAM-1, IL-6 and MCP-1 in experimental treatment group were significantly lower (P<0.05). Conclusion MiR-155 gene knockout can prevent the formation of atherosclerosis in rats, and the beneficial effect of simvastatin on the treatment of atherosclerosis in rats may provide a direction for the prevention and treatment of atherosclerosis.