Correlation among PON1 gene polymorphism, platelet reactivation and in-stent thrombosis

Liu Keqi; Yu Fengxu; Yan Jiqiang

doi:10.3969/j.issn.1674-4055.2017.08.12

Abstract：Objective To investigate the risk factors influencing the prognosis of percutaneous coronary intervention (PCI), and analyze the correlation among the gene polymorphism of serum paraoxonase (PON1), post-treatment platelet reactivation (HPPR), postoperative major adverse cardiovascular events (MACE) and in-stent thrombosis in patients undergone PCI.Methods The patients undergone PCI (n=362) were chosen from the People's Hospital of Ya'an City from Jan. 2015 to Dec. 2015. The platelet aggregation rate (PAR) was detected after PCI in all patients. The best PAR value of incidence of postoperative major adverse cardiovascular events (MACE) was predicted by using receiver operating curve (ROC). All patients were divided into HPPR group (PAR≥44.5％,n=180) and control group (PAR<44.5％,n=182) after PAR detection by using polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP). The genotypes and alleles of CYP2C19 gene at 681 (G/A) loci and their distribution were recorded in each patient. The incidence of MACE was statistically analyzed in all patients during hospitalization and 6 months after PCI.Results The incidence of MACE and in-stent thrombosis was 63.9％ at -108C/T locus, 25.0％ at -126G/C locus and 23.9％ at -162G/A locus in HPPR group, which were all significantly higher than those in control group (25.6％, 44.0％ and 12.1％,P<0.05). The genotype of -108C/T was an independent predictive factor for incidence of MACE and in-stent thrombosis (OR=3.647, 95％CI: 1.633-6.275, P=0.016).Conclusion The genotype of -108C/T and HPPR are independent predictive factors for incidence of MACE and in-stent thrombosis.