Influence of zinc ion on human umbilical vein endothelial cells
ZHANG Xiao-lei
REN Yan-chun
ZHAO Yong-feng
HU Xi-tian
XU Lei
AN Shao-bo
WU Zhi-hong
Abstract：Objective To study the influence of zinc chloride (ZnCl2) on human umbilical vein endothelial cells (HUVECs).Methods HUVECs were in vitro cultured and treated with ZnCl2 respectively in doses from 6μM to 34 μM. The growth activity was detected by using MTS test for screening the best dose of ZnCl2 to promote HUVECs proliferation as test group dose. The apoptosis of HUVECs was detected by using flow cytometry in test group and control group (without ZnCl2 treating). The cytoskeleton remodeling of HUVECs was detected after FITC-Phalloidine staining. The migration and invasiveness of HUVECs were detected by using Transwell chamber test. The relative expressions of matrix metalloproteinase-2 (MMP-2) mRNA and matrix metalloproteinase-9 (MMP-9) mRNA were detected by using real-time fluorescence quantitative polymerase chain reaction (RT-PCR). The activities of MMP-2 and MMP-9 were detected by using gelatin zymography. The levels of MMP-2 and MMP-9 in HUVECs supernatant were detected by using enzyme-linked immunosorbent assay (ELISA).Results The relative growth rate (RGR) of HUVECs treated with ZnCl2 in doses from 10 μM to 22 increased in test group compared with control group (allP<0.05). The best dose of ZnCl2 (14 μM) to promote HUVECs proliferation was chosen as test group dose. The apoptosis rate of HUVECs was (12.02±0.84)% in test group and (4.05±0.52)% in control group (P<0.01). The migration numbers of HUVECs were (112.20±10.83) in test group and (94.80±9.50) in control group (P<0.05). The invasiveness numbers of HUVECs were higher in test group than those in control group [(36.20±3.22)vs. (32.80±1.07),P<0.05]. The expressions of MMP-2 mRNA and MMP-9 mRNA of HUVECs were higher in test group than those in control group (allP<0.05). The optical density (OD) value of MMP-2 was significantly higher in test group than that in control group (P<0.05). The OD value of MMP-9 was higher in test group than that in control group (P<0.05). The absorbance values of MMP-2 and MMP-9 in HUVECs supernatant were higher in test group than those in control group (allP<0.05).Conclusion HUVECs, treated with ZnCl2 (14μM), are in an active statement of growth with the balance of proliferation and apoptosis, and their cytoskeleton remodeling and migration are promoted.
Keywords：Zinc ionHuman umbilical vein endothelial cellsProliferationApoptosisMigration
Publication Date：2017-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：5( 677-681 )