Influence of silibinin on heart function and oxidative stress in rats with myocardial ischemia-reperfusion injury

CAO Xu-dan; REN Chun-mei; HE Tian-tian; LIU Jian

doi:10.3969/j.issn.1674-4055.2017.05.16

Abstract：Objective To study the influence of silibinin (SIL) on heart function and oxidative stress in rats with myocardial ischemia-reperfusion injury.Methods SD rats (n=100) were randomly divided into sham-operation group, model group, low-dose SIL group [100 mg/(kg·d)], mid-dose SIL group [200 mg/(kg·d)] and high-dose SIL group [400 mg/(kg·d), eachn=20]. All SIL groups were give pretreatment by oral SIL 7 d before the operation, and after 7 d the rats were given ligation of coronary artery for 30 min for establishing the model of myocardial ischemia-reperfusion injury, and the reperfusion time was for 6 h. The indexes of left ventricular end-diastolic inner diameter (LVEDd), left ventricular end-systolic diameter (LVESd), fraction shortening (FS), ejection fraction (EF) and stroke volume (SV) were detected through high resolution ultrasound imaging system. The content of serum myocardial enzyme was detected by using biochemical method, changes of histo-morphology structure were observed after HE staining, levels of antioxidase and malondialdehyde (MDA) were detected by using chromatoptometry, expression of myocardial NF-κB protein was detected by using Western blotting, and myocardial apoptosis was observed by using TUNEL method. Results Compared with model group, LVEDd decreased significantly, and FS, EF and SV increased significantly, level of myocardial enzyme (AST, CPK, LDH) was significantly reduced, activities of antioxidase (SOD, GSH-Px, CAT) were promoted, and MDA level was significantly reduced, and expression of NF-κB protein was significantly reduced, myocardial apoptosis was significantly relieved, and apoptosis index (AI) was significantly reduced (P<0.05 to P<0.01) in mid-dose SIL group and high-dose SIL group.Conclusion SIL has the effects of improving heart function and inhibiting oxidative stress damage, and plays a preventive role to acute myocardial infarction in rats with myocardial ischemia-reperfusion injury.