Effect of Medicine Pre-treatment on serotonin (5-HT) in Rats of Myocardial Infarction Co-exist with Depression

LIU Mei-yan; ZHANG Li-jun

doi:10.3969/j.issn.1674-4055.2016.12.13

Abstract：Objective To explore the effect of different pre-treatments on serum 5-HT, platelet 5-HT and brain 5-HT in the Sprague-Dawley rats of myocardial infarction (MI), depression, and myocardial infarction co-exist with depression (MI + depression), and study the effect of the drug in preventing MI and depression.Methods 80 SD rats were allocated into pretreatment group and control group randomly. After 4 weeks’ pretreatment with Chinese patent medicine for benefiting qi and activing blood circulation and improving microcirculation system (include panax and ginseng etc.), then both of the two groups were established four models of shame subgroup, depression subgroup, myocardial infarction subgroup, MI + depression subgroup. The number of rats successfully modeled in control group was as follows: 8 rats in shame subgroup (80%), 9 rats in depression subgroup (90%), 8 rats in myocardial infarction subgroup (80%) and 8 rats in MI + depression subgroup (80%). The number of rats successfully modeled in pretreatment group was as follows: 9 rats in shame subgroup (90%), 10 rats in depression subgroup (100%), 9 rats in myocardial infarction subgroup (90%) and 8 rats in MI + depression subgroup (80%). All the rats were sacrificed 3 days later. 5-HT in serum, platelet, and brain of the rats in different groups were measured.Results In control group, 5-HT in serum and platelet decreased significantly in MI, depression and MI +depression subgroup compared with shame subgroup (allP<0.05); 5-HT in brain of MI subgroup was lower than the shame subgroup, while the depression and MI + depression were higher, but without any significance (allP>0.05). In pretreatment group, 5-HT in serum and brain increased significantly in MI, depression and MI + depression subgroup compared with shame subgroup (allP<0.05); 5-HT in platelet of MI, depression and MI + depression subgroup were lower than the shame subgroup (allP<0.05). In pretreatment group, 5-HT in serum and platelet of the four subgroups were significantly higher than in control group (allP<0.05); 5-HT in brain of the four subgroups were lower than the control group and there was significance (P<0.05) except depression subgroup (P>0.05).Conclusions In this study, drugs could improve the decrease of 5-HT in serum and platelet due to myocardial infarction, depression and myocardial infarction co-exist with depression, but failed to influence 5-HT in brain.