Study on the difference of cardiac gene expression at different stages of cardiac afterload in rats
TIAN Guo-xiang
LI Yan-chuan
ZHANG Wei
WANG Xiao-bing
MA Xiao-hui
WEI Wan-lin
Abstract：Objective To investigate the differential expression of cardiac whole gene at different time points after afterload increasing rats.Methods 10 of 70 male SD rats were randomly selected for the preoperative treatment, relevant parameters were collected as the baseline data; the rest of 60 rats were randomly divided into model group (2- weeks’ subgroup, 8- weeks’ subgroup, 18 weeks’ subgroup) and sham operation group (2-weeks’ subgroup, 8-weeks’ subgroup, 18 weeks’ subgroup) with 10 rats in each subgroup. Rats in the model group were treated with coarctation abdominal aorta to establish the model of cardiac afterload increase, and the abdominal aorta of the sham operation group was not constricted. Cardiac structure and hemodynamics were measured before operation, 2 weeks, 8 weeks and 18 weeks after operation by small animal ultrasound real-time imaging system and carotid artery cannulation. Then rats were sacrificed and the left ventricle was harvested. The rat cardiac RNA library was constructed. The library was sequenced using Illumina HiSeqTM 2500 / MiseqTM and the bioinformatics analysis was performed.Results At 2 weeks, 8 weeks, and 18 weeks after surgery, The mean arterial pressure, left ventricular mass, left ventricular end-diastolic dimension and left ventricular end-diastolic pressure of model group were significantly higher than those of baseline (P<0.05). Left ventricular ejection fraction (LVE) and left ventricular pressure (LVP) were significantly lower than baseline (P<0.05). There were no significant changes in mean arterial pressure, left ventricular end diastolic diameter, stroke volume, left ventricular ejection fraction, and left ventricular pressure in sham - operated rats compared with baseline (P>0.05). The rat model of afterload increase was established successfully. The number of genes differentially expressed between the model group and the sham-operated group increased at the 2nd week and the 8th week compared with the baseline, and the number of the differentially expressed genes decreased at the 18th week. Compared with the sham group, the expression of genes in the model group was significantly increased at the same time. At the 2nd week, 8th week and 18th week,the number of genes differentially expressed between the model group and the sham operation group were 119, 43 and 49 genes respectively, and the number of different genes decreased significantly with time. At different time points, there was a significant difference in gene functional enrichment (GO) and pathway enrichment (KEGG) between the model group and the sham-operated group.Conclusion Compared with the sham-operated group, the number of differentially expressed genes in the early postoperative period increased significantly, and the number of the differentially expressed genes in the middle and late post-operation decreased gradually. There were significant differences in the function of different genes and related pathways at different time points.
Keywords：HeartAfterloadGene expressionRat
Publication Date：2016-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：6( 1435-1440 )