Changes in the expression of Meox1 after acute myocardial infarction in rats
SHEN Jun
YUAN Ping
Tang Jun-ming
YANG Jian-ye
LI Xing-yuan
Zhang Lei
ZHAO Ji-xian
ZHANG Huan-xin
XUE Shi-zhen
FENG Yi
WANG Jia-ning
Abstract：Objective To analyze the expression of Meox1 in myocardium in rats with acute myocardial infarction (AMI).Methods Male SD rats (n=20) were randomly divided into AMI group and sham-operation group (eachn=10). The model of AMI was established by ligation of left anterior descending branch of coronary artery in AMI group (2 rats died during the procedure), and sham-operation group was given the same procedure except of ligation. The rats were killed 7 d after the procedure for preparing paraffin sections, and changes of myocardial tissue were observed after HE staining and Masson staining and expression of Meox1 was detected by using immunohistochemistry technique.Results The results of HE staining showed that cardiomyocyte decreased significantly at infarction zone, and there were karyorrhexis, nuclear disappearing, disorganized connective tissue in quantity, and severe infiltration of granulocytes and monocytes in AMI group. The compensatory hypertrophy and cross striatation blurring or disappearing of cardiomyocytes, widened myocardial gap edema, fibrinolysis and fragmentation of partial myocardial fibers, infiltration of inflammatory cells and proliferation of fibroblast were observed at infarction marginal zone. The normal myocardial histology, clear structure and aligned myocardial fibers were observed at infarction remote zone and in sham-operation group. The results of Masson staining showed that red was normal myocardial tissue and blue was collagen fibers at non-infarction zone. The cardiomyocytes were aligned and tight, and there were less collagen fibers in intercellular space in sham-operation group and at infarction remote zone. There was deposition of collagen fibers in quantity in intercellular space and at infarction zone and infarction marginal zone in AMI group. The results of immunohistochemistry technique showed that there was deposition of brown granules and Meox1 expression in cardiomyocytes at infarction zone and infarction marginal zone, and there was no Meox1 expression in normal cardiomyocytes at infarction remote zone and in sham-operation group. There was no Meox1 expression observed in capillary endothelial cells in sham-operation group and at infarction remote zone, and which was not significant at infarction zone and infarction marginal zone in AMI group.Conclusion The expression of Meox1 increases in myocardial tissue in AMI rats, which indicates that Meox1 expression may take part in the myocardial remodeling after AMI.
Keywords：Myocardial infarctionHomeobox gene Meox1Expression
Publication Date：2016-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：4( 1329-1332 )