Correlation between endogenous hydrogen sulfide or homocysteine and hypertension complicating aortic dissection
CAO Chun-hui
HUANG Jun
CHEN Xiao-liang
ZHU Dan
LIU Jia
LI Ren
Abstract：Objective To investigate the correlation between endogenous hydrogen sulfide (H2S) or homocysteine (Hcy) and hypertension complicating aortic dissection.Methods The patients with hypertension (n=110, male 98, female 12 and aged from 38 to 71) were chosen from the Department of Cardiology of the First Central Hospital of Chenzhou City and Guandong Provincial Cardiovascular Institute from Jun. 2012 to Apr. 2015. The patients with hypertension complicating aortic dissection were included in observation group (n=52), those without aortic dissection were included in control group (n=58), and other controls without hypertension were included in health group (n=50). The concentrations of plasma Hcy, folic acid and Vitamin B12 were detected respectively by using fluorescence polarization immunoassay (FPIA) and radioimmunoassay (RIA). The concentration of H2S in EDTA anticoagulative plasma was measured by using sensitive sulfur electrode method. The levels of cystathionine-β-synthase (CBS) and methylenetetrahydrofolate reductase (MTHFR) were detected by using enzyme-linked immunosorbent assay (ELISA). The polymorphisms of MTHFRC677T and CBS844ins68 were measured by using chain reaction-based restriction fragment length polymorphism (CR-RFLP) after extracting DNA from EDTA anticoagulative whole blood.Results The level of Hcy was significantly higher in observation group than that in control group [(18.1±3.5) μmol/Lvs. (12.4±4.7) μmol/L] and health group [(18.1±3.5) μmol/Lvs. (10.3±2.1) μmol/L, allP<0.05]. The levels of Vitamin B12 and H2S showed a descending trend in 3 groups, and were lower in observation group and control group than those in health group (allP<0.05). The frequency of CC genotype was significantly lower, and frequencies of CT genotype and TT genotype were significantly higher in observation group and control group than those in health group (allP<0.05). The frequency of C allele was 80.0%in health group, 48.1% in observation group and 44.0% in control group (allP<0.05). The detecting results of CBS844ins68 polymorphism in all groups showed that the comparison in genotypes and alleles had no statistical significance (allP>0.05). The concentration of MTHFR was higher in observation group and control group than that in health group (allP<0.05).Conclusion The concentrations of folic acid and Vitamin B12 decrease and concentration of Hcy increases in patients with hypertension complicating aortic dissection, which then induces the disorders of Hcy and H2S. It is speculated that the genetic reasons are MTHFRC677T polymorphism and T allele carried by the patients.
Keywords：Hydrogen sulfideHyperhomocysteinemiaHypertensionAortic dissection
Publication Date：2016-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：3( 1072-1074 )