Role of P38MAPK in human pulmonary microvascular endothelial cells apoptosis induced by hypoxia and inflammation combination
JI Sheng-jun
WEI Xiao-qun
MO Guan-wen
MEI Zhan-qiang
ZHANG Pei-fang
Abstract：Objective To investigate the effect of p38mitogen-actvated protein kinase (P38MAPK) on the apoptosis of human pulmonary microvascular endothelial cells induced by hypoxia and inflammation.Methods Human pulmonary microvascular endothelial cell line was recovered, subcultured, divided into blank control group, hypoxia group (hypoxia 6 h, 12 h, 24 h) and TNF alpha stimulation group (10 ng/ml, 20 ng/ml, 50 ng/ml, 100 ng/ml TNF alpha), model group (hypoxia combined with 100 ng/ml TNF alpha ) and pathway blocker group (SB203580). P38MAPK expression in each group was detected by Western blotting. Caspase-3 activity in each group was analyzed by flow cytometry. Apoptosis were detected by combination of flow cytometry and TUNEL assay.Results Compared with the blank control group, p-P38MAPK expression of model group was increased (P<0.05). Caspase-3 activity of model group (24 h hypoxia combined with 100ng/ml TNF alpha) was reduced compared with hypoxia group (24 h) and TNF alpha stimulation group (100 ng/ml) (P<0.01); Caspase-3 activity of pathway blocker group was reduced compared with model group (P<0.01). Apoptosis rate and apoptotic index were reduced in pathway blocker group than model group (P<0.01).Conclusion P38MAPK plays a role in promoting in human pulmonary microvascular endothelial cells apoptosis induced by hypoxia and inflammation. P38MAPK pathway blocker (SB203580) has protective effect on hypoxia and inflammation in human pulmonary microvascular endothelial cells.
Keywords：P38MAPKHuman pulmonary microvascular endothelial cellsApoptosis
Publication Date：2016-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：4( 676-679 )