Effect of shenxiong glucose injection on intimal hyperplasia of rabbit vascular after carotid artery balloon injury
SHI Xiu-cai
XIN Shuan-li
KONG Ling-en
SONG Xin-xing
WANG Li-min
SHI Wen-jun
Abstract：Objective To observe the effect of shenxiong glucose injection on intimal hyperplasia of rabbit vascular after carotid artery balloon injury. Methods 35 four months old healthy male New Zealand rabbits were randomly divided into control group, operation group, simvastatin group, shenxiong glucose group, shenxiong glucose+ simvastatin group. Each group had 7 rabbits. Two weeks after operation and drug intervention, serum cholesterol level were measured. Hematoxylin and eosin staining (HE) was used to observe intimal hyperplasia;immunohistochemical staining was used to analyze intimal proliferation cell nuclear antigen (PCNA) and matrix metalloproteinase-9 (MMP-9) expression, Western blot was used to analyze carotid glucose-regulated protein 78 (GRP78) expression. Results There was no different in serum cholesterol levels between operation group and control group. Serum cholesterol levels of simvastatin group was lower than control group, operation group and shenxiong glucose group (P<0.05). Serum cholesterol levels of shenxiong glucose group was lower than control group and operation group (P<0.05). Serum cholesterol levels of shenxiong glucose+ simvastatin group was lower than simvastatin group with no significant difference (P>0.05). HE result showed that intima of operation group was thicker than control group (P<0.05); intima hyperplasia of shenxiong glucose group was reduced than operation group and simvastatin group (P>0.05); intima hyperplasia of shenxiong glucose+ simvastatin group was reduced than shenxiong glucose group with no significant difference (P>0.05). Immunohistochemical analysis results showed that PCNA, MMP9 positive cells in shenxiong glucose group was reduced than operation group (P<0.05); PCNA, MMP9 positive cells in shenxiong glucose group was reduced than simvastatin group with no significant difference (P>0.05);PCNA, MMP9 positive cells in shenxiong glucose+ simvastatin group was reduced than shenxiong glucose group with no significant difference (P>0.05). GRP78 expression in shenxiong glucose group was reduced than operation group and simvastatin group according to result of Western blot (P<0.05). Conclusion The Shenxiong glucose can effectively reduce neointimal hyperplasia after balloon injury.
Keywords：Shenxiong glucose injectionBalloon endothelial injuryIntimal hyperplasia
Publication Date：2016-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：5( 619-623 )