Influence of lipopolysaccharide (LPS) on expressions of inflammatory factors of in vitro human coronary artery smooth muscle cells (HCASMC)：an investigation on establishing in-stent restenosis inflammation cell model
LI Hong-Mei
WANG Xian
Abstract：Objective To investigate the influence of lipopolysaccharide (LPS), at different time points, on expressions of inflammatory factors of in vitro human coronary artery smooth muscle cells (HCASMC), and provide the test data for the establishment of inflammation activation model after stenting.Methods HCASMC was cultured in vitro for 3-5 generations, and seeded onto 6-well plates. LPS in different doses (0.01, 0.1, 0.5, 1.0, 10) μg/mL was added at different time points (6 h, 24 h, 48 h). The expressions of interleukin-6 (IL-6), interleukin-1β (IL-1β) and tumor necrosis factor-α (TNF-α) were detected by using ELISA.Results There was only less IL-1β secreted in control group. Compared with control group, the expression of IL-1β had no significant changes in different-dose LPS groups after LPS intervention for 6 h (allP>0.05), while after LPS intervention for 24 h or 48 h, the expression of IL-1β increased significantly in different-dose LPS groups (allP<0.05). There was only less IL-6 secreted in control group. Compared with control group, the expression of IL-6 increased significantly in 0.5μg/mL group after LPS intervention for 6 h, 24 h and 48 h, and increased in 1 μg/mL group after LPS intervention for 24 h and 48 h (allP<0.05). There was only less TNF-α secreted in control group. After LPS intervention, the change trend of TNF-α was the same as that of IL-6.Conclusion LPS intervention in doses of 0.5 μg/mL and 1 μg/mL for 24 h or 48 h can improve the expressions of IL-6, IL-1β and TNF-α, which can be taken as a reference for establishing inflammation activation model after stenting.
Keywords：LipopolysaccharideHuman coronary artery smooth muscle cellsInflammatory factorsCell model
Publication Date：2016-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：5( 297-301 )