Influence of β1 receptor blocker on changes of myocardial energy metabolism and mechanism in rats after exhaustive exercise
JI Li-li
LI Xiao-yan
MENG Ke
ZHANG Guo-ming
ZHANG Hong-ming
CHEN Ying-jian
Abstract：Objective To discuss the influence of β1 receptor blocker on changes of myocardial energy metabolism and protective mechanism to stress myocardium in rats after exhaustive exercise.Methods Male Wistar rats (n=36) were randomly divided into control group, once exhaustion group and once exhaustion+metoprolol group (treatment group, eachn=12). The concentrations of myocardial ATP, plasma epinephrine (E), norepinephrine (NE) and free fatty acid (FFA) were detected after exhaustive swimming. The expression of myocardial mitochondrial uncoupling protein 2 (UCP2) was analyzed by using RT-PCR and Western blot.Results Compared with control group, the concentrations of plasma FFA, E and NE increased and myocardial ATP decreased in once exhaustion group and treatment group (P<0.05). The results of RT-PCR showed that the expression of UCP2 mRNA increased by 64%in once exhaustion group and by 43% in treatment group compared with control group (allP<0.05). The results of Western blot showed that UCP2 expression increased by 78% in once exhaustion group and by 42% in treatment group compared with control group (allP<0.05). Correlation analysis showed that concentration of plasma FFA was positively correlated to levels of plasma E and NE (r=0.93,r=0.88,P<0.01), and UCP2 expression was greatly positively correlated to concentration of plasma FFA (r=0.98,P<0.01).Conclusion The application of β1 receptor blocker can reduce adverse impact of exercise stress on myocardial energy metabolism, and relieve cardiac overload.
Keywords：Uncoupling protein 2Sudden cardiac deathβ1 receptor blockerFree fatty acidRats
Publication Date：2016-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：5( 33-37 )