Influence of hirudin on growth of in vitro human coronary artery smooth muscle cells and human coronary ;artery endothelial cells
LI Hong-Mei
WANG Xian
Abstract：Objective To investigate the influence of hirudin on growth of in vitro human coronary artery smooth muscle cells (HCASMC) and human coronary artery endothelial cells (HCAEC) and dose-dependent relation, and indentify the effectiveness and feasibility. Methods HCASMC and HCAEC cultured in vitro (3-5 generations) were respectively seeded onto 96-well plates, and then co-incubated with different doses of hirudin (6.25 mg/ml, 3.13 mg/ml, 1.56 mg/ml, 0.78 mg/ml, 0.39 mg/ml, 0.2 mg/ml, 0.1 mg/ml, 0.05 mg/ml, and 0.025 mg/ml). After 48 h, the changes of viability and inhibitory rate of HCASMC and HCAEC were detected by using MTT. Results Compared with control group, the influence of low-dose hirudin (0.025 mg/ml~0.1 mg/ml) on HCASMC proliferation had no statistical difference (all P>0.05), and that of mid-dose and high-dose of hirudin (0.2 mg/ml~6.25 mg/ml) inhibited HCASMC proliferation (all P<0.05) and the inhibitory rate increased as the increase of hirudin dose. Compared with control group, the influence of hirudin in dose of 0.025 mg/ml~3.13 mg/ml on HCAEC proliferation had no statistical difference (all P>0.05), but hirudin in dose of 0.05 mg/ml~0.2 mg/ml had improvement effect on HCAEC proliferation. The inhibition of hirudin in high dose (6.25 mg/ml) on HCAEC proliferation increased (all P<0.05) and inhibitory rate reached 44.6%. Conclusion Hirudin in mid-dose and high-dose can inhibit HCASMC growth in and dose-dependent manner, while hirudin in low-dose had no significant inhibitory effect on HCAEC growth, and can protect even improve HCAEC growth at the same time of anti-thrombosis.
Keywords：HirudinHuman coronary artery smooth muscle cellsHuman coronary artery endothelial cellsInhibitory rate
Publication Date：2015-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：4( 792-794,797 )