Preventive effect of hydroxysafflor yellow A (HSYA) on cardiomyocyte ischemia-reperfusion injury
XU Ai-bin
LIU Jian-guo
ZHANG Jian
LI Jun-Xia
HE Yu-bin
Abstract：Objective To study the preventive effect of hydroxysafflor yellow A (HSYA) on simulated cardiomyocyte ischemia-reperfusion (SI/R) injury.Methods The model of SI/R injury was established by using H9c2 cardiomyocyte. The survival rate of cardiomyocyte and level of lactate dehydrogenase (LDH) were detected, apoptosis was detected by using TUNEL staining assay, and Akt/eNOS protein expressions were detected by using Western blot test.Resutls The survival rate of H9c2 cardiomyocyte with SI/R injury increased significantly in SI/R+HSYA group compared with SI/R group [(69.7±5.8) % vs. (79.9±5.8)%, P<0.05]. The release of LDH increased in SI/R group compared with control group [(43.3±9.4) U/L vs. (129.5±11.9) U/L, P<0.05]. HSYA decreased significantly the release of LDH, which disappeared after LY29004 (PI3K inhibitor) added. The apoptosis rate of H9c2 cardiomyocyte increased significantly in SI/R group compared with control group (P<0.05). The apoptosis rate of H9c2 cardiomyocyte decreased significantly in SI/R+HSYA group compared with SI/R group, which was reversed after LY29004 added. The expression of p-Akt increased significantly in SI/R+HSYA group compared with SI/R group (P<0.05), which was significantly inhibited after LY29004 added. The expression of p-eNOS increased significantly in SI/R+HSYA group compared with SI/R group (P<0.05), which was significantly inhibited after LY29004 added.Conclusion HSYA can improve the viability of H9c2 cardiomyocyte, relieve cardiomyocyte injury, inhibit cardiomyocyte apoptosis, and play a protective role through activating Akt/eNOS signal path.
Keywords：Hydroxysafflor yellow AIschemia-reperfusion injuryCardiomyocyteApoptosis
Publication Date：2015-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：4( 663-665,671 )