Influence of medicated serum of Qiangxin Fumai Fang on action potential of injured sino-atrial node cells in neonatal rats
WANG Yan-li
LIU Ru-xiu
LIU Yu
LI Yang
LIU Jin-feng
Abstract：Objective To study the influence of medicated serum of Qiangxin Fumai Fang on action potential of injured sino-atrial node cells (SNC) in neonatal rats. Methods SNC were isolated in Wistar neonatal rats and divided into 6 groups including normal group (group 1), model group (group 2), 100 l medicated serum group (group 3), 200 l medicated serum group (group 4), 300 l medicated serum group (group 5) and blank serum group (group 6). Except of group 1, other groups were given simulating ischemia-reperfusion (I-R) for establishing cell injury model. The cell spontaneous action potential was recorded by using patch clamp technique under current clamp mode in all groups. The durations from repolarization to 20%action potential (APD20), 50%action potential (APD50) and 90%action potential (APD90), the maximal diastolic potential (MDP), and action potential amplitude (APA) were detected in all groups. Results Compared with group 2, APD20 was shortened in group 3, group 4 and group 5 [(77.2 ±5.5) ms vs. (35.7±7.1) ms], [(77.2±5.5) ms vs. (50.1±7.5) ms], [(77.2±5.5) ms vs. (39.7±4.3) ms, all P<0.01], and APD50 was shortened in group 3, group 4 and group 5 [(147.5±5.1) ms vs. (90.6±5.8) ms], [(147.5±5.1) ms vs. (111.0±4.1) ms], [(147.5±5.1) ms vs. (109.0±2.4) ms, all P<0.05]. Compared with group 1, MDP increased and APA decreased in group 2 [(-61.9±5.4) mV vs. (-54.5±4.6) mV], [(84.7±5.0) mV vs. (71.4±4.7) mV, all P<0.01]. Compared with group 2, MDP had no significant changes in group 3, group 4 and group 5 (all P>0.05). APA increased in group 3 and group 5 compared with group 2 [(83.2±5.9) mV vs. (71.4±4.7) mV], [(82.2±6.4) mV vs. (71.4±4.7) mV, all P<0.01]. Conclusion The medicated serum of Qiangxin Fumai Fang can shorten APD20 and APD50 of action potential of injured SNC, increased APA, shorten duration of action potential, and improve frequency of spontaneous beat in neonatal rats.
Keywords：Qiangxin Fumai FangMedicated serumSino-atrial node cellsPatch clampAction potentialNeonatal rats
Publication Date：2015-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：4( 444-447 )