Protective effect of levamlodipine combining valsartan on vascular endothelial function

CUI Feng-wen; SI Dao-yuan; YANG Ji-ning; YANG Ping

doi:10.3969/j.1674-4055.2015.03.35

Abstract：Objective To discuss the protective effect of levamlodipine combining valsartan on vascular endothelial function. Methods Test rats (n=24, with spontaneous hypertension) were divided into 5 groups:group A treated with levamlodipine, group B treated with valsartan, group C treated with levamlodipine combining valsartan, and group D (blank control group, each n=6), and other WKY rats (n=6) were chosen as group E (normal group). After treating and observing for 8 w, the changes of non-invasive blood pressure (NBP) and LVEF were observed. The ratio of heart weight to body weight (HW/BW), left ventricular weight to body weight (LVW/BW) were calculated after executed rats for separating myocardial tissue. The levels of endothelial nitric oxide synthase (eNOS), superoxide dismutase (SOD), reduced nicotinamide adenine dinucleotidephosphate (NADPH), interleukin-6 (L-6) and tumor necrosis factor-α (THF-α) were detected by using enzyme-linked immunosorbent assay (ELISA). Results The levels of NADPH, IL-6, THF-α, HW/BW and LVW/BW decreased significantly, and levels of SOD and eNOS increased significantly in group A, group B and group C compared with group D, and these changes were more significant in group C (177.3±3.8, 5.1±0.8, 47.9±3.1, 83.9±3.9, 127.0±9.7, 5.2±0.4, 3.3±0.2), which indicated that levamlodipine and valsartan had an interactive effect. NBP decreased in group A, group B and group C in varying degrees, and increased significantly in group D. LVEF had no significant changes in group A, group B and group C but decreased significantly in group D. Conclusion Levamlodipine and valsartan all have protective effects on vascular endothelial function, and they have an interactive effect when they are combined in clinical application.