Dynamic changes of macrophage migration inhibitory factor in myocardial ischemia/reperfusion and ;acute myocardial infarction in rabbits
ZHANG Cun
JI Xiang-wu
Abstract：Objective To study the dynamic changes of macrophage migration inhibitory factor (MIF) in myocardial ischemia/reperfusion and acute myocardial infarction (AMI) in rabbits. Methods Male New Zealand rabbits (n=18) were randomly divided into sham-operation group, AMI group and reperfusion group (each n=6). The models of reperfusion and AMI were established by using 2-suture 2-ligation method, sham-operation group was not given ligation, AMI group was given ligation of left anterior descending branch, and reperfusion group was given ligation for 30 min and reperfusion for 60 min. The blood samples were collected from femoral vein at different time points including 1 h before operation and 3 h, 6 h, 24 h, 48 h and 72 h after operation for detecting plasma MIF concentration by using enzyme-linked immunosorbent assay (ELISA). Results The plasma MIF concentration had no statistical difference among 3 groups before operation (all P>0.05), and increased in reperfusion group and AMI group 3 h, 6 h, 24 h, 48 h and 72 h after operation compared with sham-operation group (all P<0.05). Conclusion MIF will release more and its concentration will increase during the process of AMI and ischemia/reperfusion.
Keywords：Macrophage migration inhibitory factorMyocardial reperfusion injuryAcute myocardial infarctionMyocardial markersRabbits
Publication Date：2015-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：3( 320-322 )