Influences of lacidipine on expressions of endothelin and calreticulin of vascular smooth muscle cell in rats of hypertensive model
GE Ying-xiao
LI Gang
GUO Hui-xin
WANG Dong-zhi
WEI Yi-dong
Abstract：Objective To investigate the influences of lacidipine on expressions of endothelin (ET) and calreticulin (CRT) of vascular smooth muscle cell (VSMC) in rats of renal hypertensive model. Methods Male Wistar rats (n=40, aged from 7 to 8 w) were randomly divided into sham-operation group, model group, lacidipine group and positive control group (each n=10). The sham-operation group was not given and other groups were given renal artery constriction. The lacidipine group was treated with lacidipine (0.72 mg/kg/d), and positive control group was given captopril (35 mg/kg/d) once a day for 4 w before surgery. The sham-operation group and model group were given normal saline water. After 4 w, blood pressure was detected and mean arterial pressure was calculated. The expression of CRT was detected by using immunohistochemistry technique, and level of plasma ET was detected by using radioimmunoassay (RIA). Results Compared with sham-operation group, mean arterial pressure increased [(119.83±9.41) mmHg vs. (180.75±11.26) mmHg] in model group (P<0.01). Compared with model group, mean arterial pressure decreased in positive control group [(180.75±11.26) mmHg vs. (138.68±10.46) mmHg] and lacidipine group [(180.75±11.26) mmHg vs. (139.54±10.21) mmHg, all P<0.01]. Compared with sham-operation group, AOD value of CRT expression increased [(0.125±0.04) vs. (0.436±0.05)] in model group (P<0.01). Compared with model group, AOD value of CRT expression decreased in positive control group [(0.436 ±0.05) vs. (0.195±0.04)] and also decreased in lacidipine group [(0.436±0.05) vs. (0.209±0.03), all P<0.01]. Compared with sham-operation group, AOD value of ET expression increased in model group [(1.62±0.45) vs. (3.86 ±1.05), P<0.05]. Compared with model group, AOD value of ET expression decreased in positive control group [(3.86±1.05) vs. (2.04±0.55)], and decreased also in lacidipine group [(3.86±1.05) vs. (2.15±0.84), all P<0.05]. Conclusion Lacidipine can reduce mean arterial pressure and ET level, decrease CRT expression and protect VSMC through down-regulating endoplasmic reticulum stress in rats with renal hypertension.
Keywords：LacidipineEndothelinCalreticulinRats of hypertensive model
Publication Date：2015-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：3( 91-93 )