Level of coronary platelet microparticles in patients with acute coronary syndrome
LI Yi-min
HUANG Jin
LU Zhi-ping
LI Xiang-yu
HE Sheng-hu
Abstract：Objective To detect the level of coronary platelet microparticles (PMP) in patients with acute coronary syndrome (ACS) by applying flow cytometry, and investigate the relationship between PMP and development of ACS and mechanism. Methods The patients (n=92) were divided into 4 groups including group of ST-segment elevation acute myocardial infarction (STEAMI group, n=28), group of non-ST-segment elevation ACS (NSTE-ACS group, n=24), group of stable angina (SA group, n=20) and normal group (n=20). The blood samples (3 mL) were collected from coronary artery, and platelet-poor plasma was obtained after centrifugation. CD61 fluorescent antibody and standard microsphere (0.82μm) were successively added into the plasma, and relative level of PMP was detected by using flow cytometry. Results The level of PMP was higher in STEAMI group (8.9%±3.3%) and NSTE-ACS group (7.8%±2.4%) than that in normal group (4.7%±2.9%), and also higher in STEAMI group and NSTE-ACS group than that in SA group (5.9%±2.6%, all P<0.05). The comparison between STEAMI group and NSTE-ACS group, and between SA group and normal group showed that the difference had no statistical significance (all P>0.05). Conclusion The level of PMP increases significantly in patients with ACS and has no significant changes in those with stable angina, which indicates that PMP is related to the development of CHD. The mechanism may be related to the mutual activation of PMP and platelet and acceleration of atherosclerosis process. The detection of PMP level is helpful to the early diagnosis of high-risk ACS.
Keywords：Acute coronary syndromePlatelet microparticlesFlow cytometry
Publication Date：2014-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：3( 568-570 )