Protective effect of ischemic postconditioning on myocardial ischemia-reperfusion in rats with cardiac transplantation at different phases
XIAO Jun
LI Li
JIANG Zhen-Yu
SHI Ying-Chang
GUO Feng-Jie
YANG Shuang
WANG Hong-Ye
WANG De-Shui
ZHANG Wei
Abstract：Objective To investigate the protective effect of ischemic postconditioning on myocardial ischemia-reperfusion in rats with cardiac transplantation at different phases. Methods The model of cervical heterotopic heart transplantation (left heart work) was established in inbred line Lewis rats. After survived for 2 d, the rats were divided into ischemia-reperfusion group (each n=8 at 7 phases:3 h, 6 h, 12 h, 24 h, 2 d, 4 d and 7 d), ischemic postconditioning group (each n=8 at the same 7 phases as above mentioned phases) and sham-operation group (n=8). The value of serum CK-MB, and index of myocardial apoptosis and area of myocardial infarction of transplanted heart were compared after reperfusion. Results At 6 phases of 3 h, 6 h, 12 h, 24 h, 2 d and 4 d, the difference in average value of CK-MB had statistical significance between ischemia-reperfusion group and ischemic postconditioning group (all P<0.05). The value of CK-MB was significantly higher in ischemia-reperfusion group and ischemic postconditioning group than that in sham-operation group (all P<0.05). At phase of 6 h, the peak value of CK-MB was significantly lower in ischemic postconditioning group than that in ischemia-reperfusion group [(34.73±8.83) U/L vs. (52.58±10.05) U/L, P<0.01]. At 7 phases of 3 h, 6 h, 12 h, 24 h, 2 d, 4 d and 7 d, the difference in the index of myocardial apoptosis and area of myocardial infarction had statistical significance between ischemia-reperfusion group and ischemic postconditioning group (all P<0.05). The index of myocardial apoptosis was higher in ischemia-reperfusion group and ischemic postconditioning group than that in sham-operation group (all P<0.05). Conclusion Ischemic postconditioning can reduce the peak value of serum CK-MB, myocardial apoptosis and area of myocardial infarction in rats with heart transplantation within 3 h to 7 d after myocardial ischemia-reperfusion.
Keywords：Ischemic postconditioningIschemia-reperfusionon injuryHeart transplantationRats
Publication Date：2014-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：3( 104-106 )