Establishment of HPLC fingerprinting and content determination methods of eight characteristic components in water-decocted extract of Angelicae Dahuricae Radix

FENG Ting; ZHU Shuai-ming; LUO Fu-yao; MA Hao; LI Chang-wei; YANG Yu; XU Rui; GAO Jie; SHAN Jun-jie; ZHANG You-zhi

doi:10.20251/j.cnki.1003-3734.2026.12.010

Abstract：Objective:To establish the HPLC fingerprinting and quantitative method of eight characteristic components in water-decocted extract of Angelicae Dahuricae Radix,and investigate the similarities of 14 batches of Angelicae Dahuricae Radix samples.Methods:SinoChrom ODS-BP column(250 mm×4.6 mm,5 μm)was used for the fingerprinting and quantitative assay.The mobile phase was 0.025%phosphoric acid-acetonitrile under gradient elution mode.The column temperature was 35℃,the detection wavelength was 220 nm,the flow rates were 1.0 mL·min-1 for fingerprinting and 1.2 mL·min-1 for quantitative assay,respectively.Results:In the HPLC fingerprinting,there were 29 common peaks in 14 batches of Angelicae Dahuricae Radix,and eight characteristic components were identified(xanthotol,5-hydroxy-8-methoxypsoralen,oxypeucedanin hydrate,byakangelicin,xanthotoxin,bergapten,isoimpinellin,imperatorin,and phellopterin).The fingerprints of the 14 batches of Angelicae Dahuricae Radix were similar and the similarities were greater than 0.980.The linear ranges of xanthotoxol,5-hydroxy-8-methoxypsoralen,oxypeucedanin hydrate,byakangelicin,xanthotoxin,bergapten,imperatorin,and phellopterin were 3.24～162.00,2.86～143.00,3.10～155.00,2.98～149.00,3.12～156.00,3.02～151.0,3.16～158.00,and 2.30～115.00 μg·mg-1,respectively.The content ranges of xanthotoxol,5-hydroxy-8-methoxypsoralen,oxypeucedanin hydrate,byakangelicin,xanthotoxin,bergapten,imperatorin,and phellopterin were 0.14～0.37,0.26～0.39,2.31～3.48,1.09～2.22,0.04～0.26,0.19～0.34,0.03～0.14,and 0.11～0.23 μg·mg-1,respectively for the 14 batches of Angelicae Dahuricae Radix.Conclusion:The established methods of HPLC fingerprinting and quantitative analysis are reproducible,reliable,and stabile.In the future,these methods can be used for the quality control of original,processed materials and products of Angelicae Dahuricae Radix.