Memory T cells biodistribution in NCG mice
QIN Xiao-jin
DAI Cai-ling
WEN Yu-ying
GUO Jian-min
DUAN Xiao-qun
YANG Wei
Abstract：Objective:To systematically investigate the dynamic tissue distribution and tumor-targeting characteristics of memory T cells in non-tumor-bearing and tumor-bearing NCG mouse models using validated qPCR and in vivo imaging techniques.Methods:A total of 144 NCG mice were randomly divided into non-tumor-bearing control,tumor-bearing control,non-tumor-bearing treatment,and tumor-bearing treatment groups.The treatment groups received a single intravenous injection of 1×107 memory T cells per mouse,while the control groups re-ceived vehicle only.In vivo imaging was performed on days 1,3,7,14,21,and 28 post-administration.Tissues and body fluids were collected at the same time points,and the genomic distribution of memory T cells was quanti-fied by qPCR.Results:In non-tumor-bearing mice,memory T cells were initially(day 1～3)distributed mainly in highly perfused organs such as the lung,spleen,and liver,followed by a rapid decline and near clearance by day 14.In tumor-bearing mice,the early distribution pattern resembled that in non-tumor-bearing animals;howev-er,starting from day 14 onward,significant tumor enrichment was observed and persisted until day 28,accompa-nied by a concurrent decrease in cell concentrations in non-target tissues.Conclusion:Memory T cells demonstrate a trend of rapid clearance in the absence of antigenic stimulation,whereas in the presence of tumor,they exhibit antigen-driven tumor homing and long-term persistence capabilities.
Keywords：memory T cellsbiodistributiontumor targetingqPCR techniqueadoptive cell therapy
Publication Date：2026-04-30
Online Publishing Date：2026-08-14(First online date of this platform, not the publication date of the document)
Pages：7( 875-881 )