Precise in situ analysis of liposome physical stability based on cryo-electron microscopy
LIU Jing
YAN Meng-yu
HAN Yao
YU Ya
HE Lin
GUO Xin-qiu
Abstract：This study employs cryo-electron microscopy(Cryo-EM)to precisely evaluate the physical sta-bility of liposomes in situ.Cryo-EM eliminates the need for drying and staining,thereby preserving the native mor-phology of liposomes to the greatest extent.It clearly reveals the bilayer membrane structure and the spatial distri-bution of encapsulated substances,overcoming issues such as deformation,hydration layer interference,and probe convolution effects associated with traditional characterization methods.Cryo-EM enables efficient and intuitive as-sessment of liposome morphology,particle size,size distribution,and the state of encapsulated materials.Cryo-EM provides reliable scientific evidence and quality control standards for the development and optimization of liposomal formulations.Ultimately,it enhances the consistency and comparability of drug development and production,meet-ing the growing clinical demands.
Keywords：liposomescryo-electron microscopymorphologyparticle sizecritical quality attributes
Publication Date：2026-04-30
Online Publishing Date：2026-08-14(First online date of this platform, not the publication date of the document)
Pages：6( 869-874 )