Simultaneous determination of 10 components in Puqin Jiedu Lishi granules by HPLC-PDA method
LI Shuai-yin
XI Hui-rong
CHEN Ting
LI Xi-xiang
LI Xiao-feng
ZHOU Ya-li
YANG Shou-yuan
Abstract：Objective:To establish an HPLC-PDA method for the simultaneous determination of 5-hydroxy-methylfurfural,chlorogenic acid,caffeic acid,isochlorogenic acid A,cichoric acid,baicalin,oroxylin A-7-O-β-D-glucuronide,wogonoside,baicalein and glycyrrhizic acid in the Puqin Jiedu Lishi Granules.Methods:The analysis was performed on a Waters Symmetry C18 column(250 mm×4.6 mm,5 μm)with gradient elution using acetonitrile and 0.2%phosphoric acid solution as the mobile phase.The flow rate was 1.0 mL·min-1,the column temperature was 20℃,and the detection wavelengths were set at 284 nm,327 nm,280 nm and 254 nm.Results:The linear ranges of 5-hydroxymethylfurfural,chlorogenic acid,caffeic acid,isochlorogenic acid A,cichoric acid,baicalin,oroxylin A-7-O-β-D-glucuronide,wogonoside,baicalein and glycyrrhizic acid were 1.860～59.64,5.425～173.6,1.980～63.36,1.890～60.24,5.990～191.6,33.30～1066,6.440～206.0,28.00～896.0,2.044～65.40,6.660～213.1 μg·mL-1,respectively,with the correlation coefficients r≥0.999 5.The precision,stability,and reproducibility tests yielded satisfactory results.The average recoveries of the 10 components ranged from 98.15%～102.21%,with RSDs between0.96%～1.91%.Conclusion:This method is reliable,stable and reproducible,providing a reference for the quality control of Puqin Jiedu Lishi Granules.
Keywords：Puqin Jiedu Lishi granulesHPLCcontent determinationquality control
Publication Date：2026-04-15
Online Publishing Date：2026-08-14(First online date of this platform, not the publication date of the document)
Pages：7( 734-740 )